FSH Receptor and Ki-67 Protein Expressions of Endometrial Macaque in Mid-Luteal Phase after Exposure to Various Doses of Recombinant FSH and Fixed-Dose of GnRH Agonist

Online journal of biological sciences

article

Nurhuda Sahar¹  Vivitri Dewi Prasasty²  Ponco Birowo¹  Rosalina Thuffi³  Andhea Debby Pradhita³  Wa Ode Zulhulaifah³  Raden Muharram⁴  Rijal Subahar¹  Fatmawaty Yazid⁵  Supri Irianti Handayani⁶ 

[1] Department of Medical Biology, Faculty of Medicine, Universitas Indonesia;Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia;Master Program of Biomedical Sciences, Faculty of Medicine, Universitas Indonesia;Department of Obstetrics and Gynecology Faculty of Medicine, Universitas Indonesia;Department of Medical Chemistry, Faculty of Medicine, Universitas Indonesia;Department of Anatomical Pathology, Faculty of Medicine, Universitas Indonesia

关键词: FSHR;    rFSH;    Endometrial Tissue;    Proliferation Marker;    Macaque;   

DOI  :  10.3844/ojbsci.2021.260.266

学科分类：生物科学（综合）

来源: Science Publications

【 摘 要 】

There are many advances in Assisted Reproductive Technologies (ARTs). However, implantation rates are still low. The embryo implantation is a mutual interaction between the blastocyst and endometrium. This study aimed to investigate the endometrial cell proliferation stimulated by recombinant Follicle-Stimulating Hormone (rFSH) in various doses by looking at FSH-Receptor (FSHR) and Ki-67 protein expressions. The studied samples were Stored Biological Materials (SBMs) from the endometrial tissue of M acaca nemestrina . All sample was obtained from fifteen macaques, divided into four groups, which three groups were treated with rFSH and given fixed-dose (160 µg/day) of Gonadotropin-Releasing Hormone (GnRH) agonists and one control group as normal group. The three treated macaque groups were stimulated with 30, 50 and 70 IU doses of rFSH. There was no significant difference in various doses of rFSH towards FSHR and Ki-67 expressions in endometrial tissue of M. nemestrina . Therefore, this result did not significantly correlate FSHR and Ki-67 expressions in the endometrial tissue of M. nemestrina after rFSH administration. Moreover, the highest rFSH dose did not reduce the FSHR and Ki-67 expressions and there was no correlation between FSHR and Ki-67 expressions. It can be concluded that macaque endometrial cells were receptive to the given dosages of rFSH.

【 授权许可】

CC BY