LncRNA MALAT1 exhibits positive effects on nucleus pulposus cell biology in vivo and in vitro by sponging miR-503

BMC Molecular and Cell Biology

article

Zheng, Hongyu¹  Wang, Tingting²  Li, Xiangmin³  He, Wei⁴  Gong, Zhiqiang⁵  Lou, Zhenkai⁵  Wang, Bing⁵  Li, Xingguo⁵ 

[1] Department of Emergency Medical, First Affiliated Hospital of Kunming Medical University;Department of Geriatrics, Yan’ An Hospital of Kunming City;Department of Panicaceae, First Affiliated Hospital of Kunming Medical University;Department of Orthopedics, Qianjiang Central Hospital;Department of Orthopedics, First Affiliated Hospital of Kunming Medical University

关键词: Intervertebral disc degeneration;    Long noncoding RNA MALAT1;    microRNA-503;    Nucleus pulposus cells;    Apoptosis;    MAPK pathway;   

DOI  :  10.1186/s12860-020-00265-2

学科分类：内科医学

来源: Colegio Oficial de Psicologos

【 摘 要 】

Intervertebral disc degeneration (IDD) is characterized by the loss of nucleus pulposus cells (NPCs) and phenotypic abnormalities. Accumulating evidence suggests that long noncoding RNAs (lncRNAs) are involved in the pathogenesis of IDD. In this study, we aimed to investigate the functional effects of lncRNA MALAT1 on NPCs in IDD and the possible mechanism governing these effects. We validated the decreased expression of MALAT1 in the IDD tissues, which was associated with decreased Collagen II and Aggrecan expression. In vitro, overexpressed MALAT1 could attenuate the effect of IL-1β on NPC proliferation, apoptosis, and Aggrecan degradation. In vivo, MALAT1 overexpression attenuated the severity of disc degeneration in IDD model rats. Our molecular study further demonstrated that MALAT1 could sponge miR-503, modulate the expression of miR-503, and activate downstream MAPK signaling pathways. The effects of MALAT1 on NPCs were partially reversed/aggregated by miR-503 mimics/inhibitor treatment. Our data suggested that the MALAT1-miR-503-MAPK pathway plays a critical role in NPCs, which may be a potential strategy for alleviating IDD.

【 授权许可】

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