Bromide impairs the circadian clock and glycolytic homeostasis via disruption of autophagy in rat H9C2 cardiomyocytes

BMC Molecular and Cell Biology

article

Jiang, Yicheng¹  Gu, Yang¹  Xu, Hai¹  Tian, Xiaoyi¹  Zhang, Xuefeng¹  Xu, Xiaojin¹  Yan, Wenting¹  Zhang, Xiwen¹ 

[1] The Affiliated Huai’an No.1 People’s Hospital of Nanjing Medical University

关键词: Bromide;    H9C2;    Circadian clock;    Glycolysis;    Autophagy;   

DOI  :  10.1186/s12860-020-00289-8

学科分类：内科医学

来源: Colegio Oficial de Psicologos

【 摘 要 】

Trace elements function as essential cofactors that are involved in various biochemical processes in mammals. Autophagy is vital for nutrient supplement, which is an important Zeitegber for the circadian homeostasis in heart. Here, we considered the possibility that autophagy, as well as the cardiomyocyte clock and glycolysis are interlinked. Detrimental effects were observed when cardiac system is exposed to bromine containing drugs. This study investigated the effects and mechanisms of bromide on the circadian clock and glycolytic metabolism of H9C2 cardiomyocytes. In the present study, bromide does not affect cell viability and apoptosis of H9C2 cardiomyocytes. Bromide dampens the clock and glycolytic (Hk2 and Pkm2) gene expression rhythmicity in a dose-dependent manner. Additionally, bromide inhibits autophagic process in H9C2 cardiomyocytes. In contrast, rapamycin (an autophagy inducer) dramatically restores the inhibitory effect of NaBr on the mRNA expression levels of clock genes (Bmal1, Cry1 and Rorα) and glycolytic genes (Hk2 and Pkm2). Our results reveal that bromide represses the clock and glycolytic gene expression patterns, partially through inhibition of autophagy.

【 授权许可】

CC BY|CC0