Curcumin suppresses oxidative stress via regulation of ROS/NF-κB signaling pathway to protect retinal vascular endothelial cell in diabetic retinopathy

Molecular & Cellular Toxicology

article

Huang, Jiang¹  Zou, Weijie¹  Ji, Fangfang¹  Luo, Weifeng²  Yi, Quanyong³  You, Yuhong¹  Chen, Yao¹  Niu, Tongtong¹  Li, Yi⁴  Zhang, Ji¹  Ji, Xiaoyan¹  Xu, Guoxu¹ 

[1] Department of Ophthalmology, Second Affiliated Hospital of Soochow University;Department of Neurology, Second Affiliated Hospital of Soochow University;Department of Ophthalmology, Ningbo Eye Hospital;Department of Ophthalmology, Huashan Hospital, Fudan University

关键词: Curcumin;    Oxidative stress;    ROS/NF-κB;    Diabetic retinopathy;    Retinal vascular endothelial cells;   

DOI  :  10.1007/s13273-021-00144-7

来源: Korean Society of Toxicogenomics and Toxicoproteomics

【 摘 要 】

Background The retinal vascular endothelial cells can be damaged by oxidative stress even in the early stage of diabetic retinopathy (DR). This study aimed to investigate the protective effect of curcumin on the rat retinal vascular endothelial cells (RRVECs) in high glucose circumstance. Objective The cultured RRVECs were identified and characterized by both of vWF and CD31 immunofluorescence expression. The activation of ROS/NF-κB signal pathway was examined by electrophoretic Mobility Shift Assay (EMSA), immunohistochemistry and Western blot; the apoptosis of RRVECs was tested by flow cytometry. Results We found that curcumin reduced the reactive oxygen species (ROS) and relieved the apoptosis in RRVECs exposed to the high glucose by flow cytometry. It was revealed that the increased activity of NF-κB and phosphorylated NF-κB in RRVECs induced by high glucose concentration was significantly suppressed by curcumin. Conclusion We concluded that curcumin could suppress the oxidative stress via regulation of NF-κB signal to protect the RRVECs in DR.

【 授权许可】

Unknown