Aqueous extract of Phragmites commun is rhizomes attenuates phototoxicity in skin cells

Molecular & Cellular Toxicology

article

Kim, Sung Hyeok¹  Sohn, Eun-Hwa¹  Ha, Chang Woo¹  Lim, Hyosun²  Jang, Sohee¹  Namkoong, Seung¹  Hong, Sungsil¹  Kim, Youn Kyu²  Kim, Jae-Young³  Lee, Sung Ryul⁴ 

[1] College of Health Sciences, Kangwon National University;Korea Research Institute BioScience Co., Ltd;Graduate School of Analytical Science and Technology (GRAST), Chungnam National University;Department of Convergence Biomedical Science, Cardiovascular and Metabolic Disease Center, College of Medicine, Inje University

关键词: Extracellular matrix;    Photoprotection;    Phragmites communis;    Photoaging;    Skin;    Ultraviolet B irradiation;   

DOI  :  10.1007/s13273-020-00106-5

来源: Korean Society of Toxicogenomics and Toxicoproteomics

【 摘 要 】

Background Excessive sunlight exposure leads to photodamaged skin, resulting in wrinkles, roughness, relaxation, and pigmentation. We examined photoprotective effects of aqueous extracts of dried Phragmites communis rhizome (PCWE) on ultraviolet B radiation (UVB)-mediated photodamage in skin cells. Methods Human dermal fibroblasts (HDFs), melanocytes (B16F10 cells), and keratinocytes (HaCaT cells) were treated with PCWE (25–200 μg/mL), with or without UVB (30 mJ/cm2). Cell viability, cell senescence, and mRNA levels of genes involved in skin homeostasis were assessed. Anti-melanogenic effects of PCWE on B16F10 cells were evaluated. Cyclooxygenase-2 (COX-2) mRNA levels and β-hexosaminidase release were evaluated in macrophage RAW264.7 and basophilic leukemia RBL-2H3 cells, respectively. Results No significant cytotoxicity was observed in tested cells up to 200 μg/mL PCWE. In HDFs and HaCaT cells, PCWE pretreatment afforded significant, concentration-dependent photoprotection. PCWE downregulated baseline matrix metalloprotease-1 expression and elastase activity in HDFs; in HaCaT cells, telomerase reverse transcriptase and hyaluronan synthase-2 expressions were upregulated. PCWE suppressed α-melanocyte-stimulating hormone-mediated increase in melanin production and tyrosinase activity. PCWE suppressed COX-2 induction (in RAW264.7) and β-hexosaminidase release (in RBL-2H3). Conclusion PCWE exhibits good potential to attenuate photodamage in skin cells.

【 授权许可】

Unknown