Mitochondrial DNA content and deletion ratio are associated with metabolic syndrome in a general population exposed to pesticide

Molecular & Cellular Toxicology

article

Choi, Jung Ran¹  Park, Sungjin²  Kim, Sung-Kyung⁴  Kim, Jae-Yeop⁴  Lee, Kyungsuk⁶  Oh, Sung-Soo⁴  Koh, Sang Baek¹ 

[1]Institute of Genomic Cohort, Yonsei University Wonju College of Medicine

[2]Yonsei University Graduate School

[3]Department of Occupational and Environmental Medicine, Hongseong Medical Center

[4]Department of Occupational and Environmental Medicine, Yonsei University Wonju College of Medicine

[5]Department of Preventive Medicine and Institute of Occupational and Environmental Medicine, Yonsei University Wonju College of Medicine

[6]National Academy of Agricultural Science, Rural Development Administration

[7]Center for Global Health and Social Medicine, Institute of Poverty Alleviation and International Development, Yonsei University

关键词: mtDNA copy number;    Deletion ratio;    Metabolic syndrome;    Pesticide exposure;   

DOI  :  10.1007/s13273-020-00079-5

来源: Korean Society of Toxicogenomics and Toxicoproteomics

【 摘 要 】

Background Mitochondrial DNA (mtDNA) copy number and deletion ratio may be asymmetrically determined using physiologic or pathologic conditions. Mitochondrial dysfunction is associated with an increased risk of developing metabolic syndrome (MetS). Objective Here, we investigated the association between mtDNA copy number and/or deletion ratio and the risk of developing MetS in a general population exposed to the pesticide. We examined 215 randomly sampled adults who were exposed to pesticide but did not present with MetS in a prospective cohort study. Both mtDNA copy number and deletion ratio were determined using quantitative real-time polymerase chain reaction. Results During the average 2.8-year follow-up period, 76 (35.3%) participants developed new-onset MetS. The numbers of mtDNA copies were significantly lower in participants with new-onset MetS than in those without MetS (112.15 ± 26.15 vs. 133.13 ± 44.44, p < 0.001), whereas the mtDNA deletion ratios were higher in participants with MetS than in those without MetS (10.59 ± 11.96 vs. 4.52 ± 6.74, p < 0.001). In the multivariate-adjusted models, the participants with a higher tertile of mtDNA copy number were 0.355 times more likely to develop MetS than those with the lowest tertile [odds ratio (OR) 0.355, 95% confidence interval (CI) 0.150–0.841, p = 0.004]. A higher mtDNA deletion ratio was significantly associated with the risk of developing MetS in populations exposed to pesticide (OR 5.062, 95% CI 1.164–22.004, p = 0.004). Conclusion A lower mtDNA copy number and higher deletion ratio were independent predictors for new-onset MetS in a general population exposed to the pesticide.

【 授权许可】

Unknown