Toxicoproteomic analysis of deltamethrin exposure in neuroblastoma cell lines

Molecular & Cellular Toxicology

article

Kang, Min-Joo¹  Lee, Mi-Young¹ 

[1] Department of Medical Science, Soonchunhyang University;Department of Medical Biotechnology, Soonchunhyang University

关键词: Deltamethrin;    Neuroblastoma cells;    Proteomics;   

DOI  :  10.1007/s13273-019-00064-7

来源: Korean Society of Toxicogenomics and Toxicoproteomics

【 摘 要 】

Background Deltamethrin, a type II pyrethroid insecticide, has been widely used due to its high efficacy against insects and low toxicity to mammals. However, recently, potential adverse health effects during deltamethrin exposure have been reported. Methods A proteomic analysis using two-dimensional electrophoresis with mass spectrometry was performed, and the proteomic data were validated by western blotting. Antioxidant enzyme activity was also measured by spectrophotometric analysis. Results The 13 differentially expressed proteins, of which 8 were up-regulated and 5 down-regulated, are involved in a variety of cellular functions including protein folding, cytoskeleton regulation, and splicing process. The activities of antioxidant enzymes and acetylcholinesterase were also altered by deltamethrin exposure. Conclusion Differential protein expression caused by deltamethrin exposure in neuroblastoma cell lines could allow us to develop biomarkers for deltamethrin toxicity, and pursue future studies to assess the molecular mechanisms of deltamethrin toxicity.

【 授权许可】

Unknown