Serum metabolomics in mice after paraquat posioning

Molecular & Cellular Toxicology

article

Gao, Lina¹  Wang, Guang²  Yuan, Huiya¹  Xu, Enyu¹  Liu, Guojie³  Liu, Junting¹ 

[1] School of Forensic Medicine, China Medical University;Department of Laboratory Animal Center, China Medical University;School of Fundamental Sciences, China Medical University

关键词: Paraquat;    Toxicology;    Metabolomics;    Gas chromatography-mass spectrometry;   

DOI  :  10.1007/s13273-019-0049-1

来源: Korean Society of Toxicogenomics and Toxicoproteomics

【 摘 要 】

Backgrounds Paraquat is toxic to humans and there is no antidote for paraquat poisoning. Moreover, the mechanisms of paraquat toxicity have not been clearly defined. Methods In this study, we developed a serum metabolomic method using gas chromatography- mass spectrometry (GC-MS) to evaluate the effects of paraquat poisoning on mice. Pattern recognition analysis, including both principal component analysis and cross orthogonal partial least squares-discriminate analysis revealed that paraquat poisoning induced energy/redox cycle and metabolic perturbations. Results Compared with the control group, the levels of creatinine, citric acid, succinic acid, fumaric acid and glycine in the sub acute paraquat poisoning group increased (P < 0.05), while the levels of alpha-Tocopherol, 6-phosphogluconic acid and palmitoleic acid decreased (P < 0.05). Conclusion These findings provide an overview of systematic responses to paraquat exposure and metabolomic insight into the toxicological mechanism of paraquat. Our results demonstrate that the metabolomic method based on GC-MS can be a useful tool to elucidate the mechanism of PQ toxicity, and provide a new direction for its clinical diagnosis and treatment.

【 授权许可】

Unknown