The adaptive immune response in cardiac arrest resuscitation induced ischemia reperfusion renal injury

Journal of Biological Research

article

Tsivilika, Maria¹  Kotzampassi, Katerina²  Papamitsou, Theodora³  Doumaki, Eleni⁴  Stavrou, George²  Sioga, Antonia³  Grosomanidis, Vasilis²  Meditskou, Soultana³  Maranginos, Athanasios⁶  Tsivilika, Despina⁶  Stafylarakis, Dimitrios⁷ 

[1] Faculty of Medicine, School of Health Sciences, Aristotle University of Thessaloniki;Department of Surgery, AHEPA University Hospital, Aristotle University of Thessaloniki;Laboratory of Histology- Embryology, Faculty of Medicine, School of Health Sciences, Aristotle University of Thessaloniki;1st Department of Internal Medicine, Faculty of Medicine, AHEPA University Hospital, Aristotle University of Thessaloniki;Department of Colorectal Surgery, Addenbrooke’s Hospital;Independent Researcher;2nd Department of Urology of Aristotle University of Thessaloniki, Papageorgiou General Hospital

关键词: Kidney;    Ischemia reperfusion;    Cardiac arrest;    Renal injury;    Acute tubular necrosis;    Immunohistochemistry;    Adaptive immune response;   

DOI  :  10.1186/s40709-020-00125-2

学科分类：社会科学、人文和艺术（综合）

来源: Springer

【 摘 要 】

The present study aims to investigate, immunohistochemically, the role of the adaptive immune response in cardiac arrest/resuscitation-induced ischemia–reperfusion renal injury (IRI), namely to assess the presence of lymphocytes in renal tissue samples and the connection between the extent of the damage and the concentration of the lymphocytes by comparing the kidneys of non resuscitated swine with the kidneys of resuscitated swine. Twenty four swine underwent cardiac arrest (CA) via a pacemaker wire. After 7 min, without any intervention, Cardiopulmonary Resuscitation, CPR, was commenced. Five min after CPR was commenced advanced life-support, ALS. Animals were divided into resuscitated animals and non resuscitated animals. Tissue samples obtained from the two groups for immunohistological study aiming to detect T-cells, B-cells and plasma cells using CD3 + , CD20 + , and CD138 + antibodies. There seems to be a strong concentration of T lymphocytes in the kidney tissues after ischemia of both non-resuscitated and resuscitated swine. B lymphocytes, also, appear to have infiltrated the ischemic kidneys of both animal groups; nevertheless, the contribution of T lymphocytes to the induction of injury remains greater. There is no strong evidence of correlation between the plasma cells and the damage. The adaptive immune response seems to have a strong association with kidney injury and acute tubular necrosis after cardiac arrest/ resuscitation-induced ischemia–reperfusion. However, the extent to which the adaptive immune cells are involved in the induction of renal injury remains uncertain and there are many questions about the mechanism of function of these cells, the answers of which require further studies.

【 授权许可】

CC BY-NC