Cell Cycle Markers in the Evaluation of Bladder Cancer

Pathology oncology research: POR

article

da Silva, Jéssica Niederauer Leote¹  Ranzi, Alana Durayski¹  Carvalho, Caroline Trainotti¹  Scheide, Tales Vicente¹  Strey, Yuri Thomé Machado¹  Graziottin, Túlio Meyer¹  Bica, Claudia Giuliano¹ 

[1] Research Laboratory of Pathology, Health Sciences Federal University of Porto Alegre (UFCSPA);Santa Rita Hospital, Hospital system of the Porto Alegre Holy House of Mercy

关键词: Bladder cancer;    Cell cycle markers;    Prognosis;    Immunohistochemistry;   

DOI  :  10.1007/s12253-018-0389-5

来源: Springer

【 摘 要 】

Bladder cancer (BC) is a heterogeneous neoplasia characterized by a high number of recurrences. Standardized clinical and morphological parameters are not always sufficient to predict individual tumor behavior. The aim of this study was to evaluate the expression of cell cycle regulators proteins as potential adjuvant in prognosis and monitoring of this disease. Block paraffin samples from patients with urothelial bladder carcinoma treated by transurethral resection (TUR) were collected to immunohistochemistry analysis for proteins p16, p21, p27, p53, pRb and Ki-67. Chisquare, logistic regression and Kaplan-Meier curve were used to analyze the prognostic value of these markers. Of the 93 patients included in the study, the main categories of staging observed were T1 (53%) and Ta (29%), and the distribution between tumor grades was 58% of patients with low grade to 42% of patients with high grade. The expressions of p16, p21, p27, p53, pRb and Ki-67 were altered in 31%, 42%, 60%, 91%, 27% and 56% of patients, respectively. The immunohistochemical expression of Ki-67 was associated with tumor histological grade (p = 0.016), and expression of pRb with recurrence-free survival (p = 0.035), but no isolated marker was significant associated with recurrence and progression in multivariate analysis. More than two markers abnormally expressed were associated with presence of recurrence (p = 0.005) and lower recurrence-free surviva (p = 0.004). Our panel marker has important prognostic value for BC, especially when more than two have altered expression predicting good clinical recurrence implication.

【 授权许可】

Unknown