CDX1/2 and KLF5 Expression and Epigenetic Modulation of Sonic Hedgehog Signaling in Gastric Adenocarcinoma

Pathology oncology research: POR

article

Samadani, Ali Akbar¹  Nikbakhsh, Novin³  Taheri, Hassan⁴  Shafaee, Shahriyar⁵  Fattahi, Sadegh¹  Pilehchian Langroudi, Maryam¹  Hajian, Karimollah⁶  Akhavan-Niaki, Haleh¹ 

[1] Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences;Guilan University of Medical Sciences;Department of Surgery, Cancer Research Center, Babol University of Medical Sciences;Department of Gastroenterology, Babol University of Medical Sciences;Department of Pathology, Babol University of Medical Sciences;Department of Social medicine and statistics, Faculty of Medicine, Babol University of Medical Sciences;Department of Genetics, Faculty of Medicine, Babol University of Medical Sciences

关键词: Gastric cancer;    DNA methylation;    Gene expression;    Epigenetics;   

DOI  :  10.1007/s12253-019-00594-4

来源: Springer

【 摘 要 】

Gastric cancer is among the commonplace causes of cancer death worldwide. Sonic hedgehog (Shh) signaling is an important pathway which may be dysregulated in many cancers.CDX1/2, and KLF5are key transcription factors involved in Shh pathway and cancer stem cells. The aim of this study was to investigate the expression and epigenetic alterations of these genes in gastric cancer patients. DNA methylation’s modifications of CDX1, KLF5 and CDX2 genes alongside with the expressions of these genes in gastric cancer tissues and their non-tumoral counterparts (margin tissues) were analyzed using methylation specific sequencing, and Real time PCR Taq man assays, respectively. The expression of CDX1 (P = 0.002) and KLF5 (P = 0.010) were decreased significantly, but it was considerably increased for CDX2 (P = 0.001). Relatively, the results for the regulatory region methylation status of each CpG site had shown a notable fluctuation in these genes with no significant difference in most places. The creation of metastatic lymph nodes in patients was significantly associated with increased expression of CDX2 gene. The modifications of these genes expression can be considered as a cancer biomarker in future studies. Methylation of the investigated genes is not the main mechanism of gastric cancer development.

【 授权许可】

Unknown