Chicken or the Egg: Microbial Alterations in Biopsy Samples of Patients with Oral Potentially Malignant Disorders

Pathology oncology research: POR

article

Decsi, Gabor¹  Sonkodi, Istvan¹  Urban, Edit²  Nemeth, Istvan Balazs³  Nagy, Katalin¹  Buzas, Krisztina⁴  Soki, Jozsef²  Pap, Bernadett⁴  Dobra, Gabriella⁴  Harmati, Maria⁴  Kormondi, Sandor⁶  Pankotai, Tibor⁷  Braunitzer, Gabor⁸  Minarovits, Janos⁵ 

[1] Faculty of Dentistry, Department of Oral Surgery, University of Szeged;Albert Szent-Gyorgyi Clinical Centre, Institute of Clinical Microbiology, University of Szeged;Department of Dermatology and Allergology, University of Szeged;Biological Research Centre, Hungarian Academy of Sciences;Faculty of Dentistry, Department of Oral Biology and Experimental Dental Research, University of Szeged;Albert Szent-Gyorgyi Clinical Centre, Department of Traumatology, University of Szeged;Department of Biochemistry and Molecular Biology, University of Szeged;dicomLAB Ltd.

关键词: OPMD;    Oral microbiome;    Metagenome sequencing;    Lichen;    Leukoplakia;   

DOI  :  10.1007/s12253-018-0457-x

来源: Springer

【 摘 要 】

Oral carcinogenesis often leads to the alteration of the microbiota at the site of the tumor, but data are scarce regarding the microbial communities of oral potentially malignant disorders (OPMDs). Punch biopsies were taken from healthy and non-healthy mucosa of OPMD patients to analyze the microbiome using metagenome sequencing. In healthy oral mucosa biopsies the bacterial phyla Firmicutes, Fusobacteria, Proteobacteria, Actinobacteria and Bacteroidetes were detected by Ion Torrent sequencing. The same phyla as well as the phyla Fibrobacteres and Spirochaetes were present in the OPMD biopsies. On the species level, there were 10 bacterial species unique to the healthy tissue and 35 species unique to the OPMD lesions whereas eight species were detected in both samples. We observed that the relative abundance of Streptococcus mitis decreased in the OPMD lesions compared to the uninvolved tissue. In contrast, the relative abundance of Fusobacterium nucleatum, implicated in carcinogenesis, was elevated in OPMD. We detected markedly increased bacterial diversity in the OPMD lesions compared to the healthy oral mucosa. The ratio of S. mitis and F. nucleatum are characteristically altered in the OPMD lesions compared to the healthy mucosa.

【 授权许可】

Unknown