Differential Expression Study of Lysine Crotonylation and Proteome for Chronic Obstructive Pulmonary Disease Combined with Type II Respiratory Failure

Canadian Respiratory Journal

article

Qing Gan¹  Yingyun Fu²  Yong Dai¹  Donge Tang³  Qiang Yan⁴  Jiejing Chen¹  Yong Xu³  Wen Xue¹  Lu Xiao²  Fengping Zheng³  Huixuan Xu³ 

[1]Guangxi Key Laboratory of Metabolic Disease Research, Department of Clinical Laboratory of Guilin No. 924 Hospital

[2]Key Laboratory of Shenzhen Respiratory Diseases, Department of Pulmonary and Critical Care Medicine, Shenzhen Institute of Respiratory Disease, The First Affiliated Hospital of Southern University of Science and Technology, The Second Clinical Medical College, Jinan University, Shenzhen People’s Hospital

[3]Clinical Medical Research Center, The Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital

[4]Organ Transplantation Center of Guilin No. 924 Hospital

DOI  :  10.1155/2021/6652297

学科分类：工程和技术（综合）

来源: Hindawi Publishing Corporation

【 摘 要 】

Introduction . The modification of lysine crotonylation (Kcr) is another biological function of histone in addition to modification of lysine acetylation (Kac), which may play a specific regulatory role in diseases. Objectives . This study compared the expression levels of Kcr and proteome between patients with chronic obstructive pulmonary disease (COPD) combined with type II respiratory failure (RF) to study the relationship between Kcr, proteome, and COPD. Methods . We tested the Kcr and proteome of COPD combined with type II RF and normal control (NC) using croton acylation enrichment technology and liquid chromatography tandem mass spectrometry (LC-MS/MS) with high resolution. Results . We found that 32 sites of 23 proteins were upregulated and 914 sites of 295 proteins were downregulated. We performed Kyoto Encyclopedia of Genes and Genomes (KEGG), protein domain, and Gene Ontology (GO) analysis on crotonylated protein. In proteomics research, we found that 190 proteins were upregulated and 151 proteins were downregulated. Among them, 90 proteins were both modified by differentially expressed crotonylation sites and differentially expressed in COPD combined with type II RF and NC. Conclusion . Differentially expressed crotonylation sites may be involved in the development of COPD combined with type II RF. 90 proteins modified by crotonylation and differentially expressed in COPD combined with type II RF can be used as markers for the study of the molecular pathogenesis of COPD combined with type II RF.

【 授权许可】

CC BY