Cortico-autonomic local arousals and heightened somatosensory arousability during NREMS of mice in neuropathic pain

eLife

Sandro Lecci¹  Anita Lüthi¹  Alejandro Osorio-Forero¹  Laura MJ Fernandez¹  Romain Cardis²  Isabelle Decosterd³  Paul Chu Sin Chung³  Stephany Fulda⁴ 

[1] Department of Fundamental Neurosciences, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland;Department of Fundamental Neurosciences, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland;Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV), Lausanne, Switzerland;Pain Center, Department of Anesthesiology, Lausanne University Hospital (CHUV), Lausanne, Switzerland;Sleep Medicine Unit, Neurocenter of Southern Switzerland, Civic Hospital (EOC) of Lugano, Lugano, Switzerland;

关键词: NREMS;    spared-nerve-injury;    arousal;    insomnia;    gamma power;    infraslow;    Mouse;   

DOI  :  10.7554/eLife.65835

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Frequent nightly arousals typical for sleep disorders cause daytime fatigue and present health risks. As such arousals are often short, partial, or occur locally within the brain, reliable characterization in rodent models of sleep disorders and in human patients is challenging. We found that the EEG spectral composition of non-rapid eye movement sleep (NREMS) in healthy mice shows an infraslow (~50 s) interval over which microarousals appear preferentially. NREMS could hence be vulnerable to abnormal arousals on this time scale. Chronic pain is well-known to disrupt sleep. In the spared nerve injury (SNI) mouse model of chronic neuropathic pain, we found more numerous local cortical arousals accompanied by heart rate increases in hindlimb primary somatosensory, but not in prelimbic, cortices, although sleep macroarchitecture appeared unaltered. Closed-loop mechanovibrational stimulation further revealed higher sensory arousability. Chronic pain thus preserved conventional sleep measures but resulted in elevated spontaneous and evoked arousability. We develop a novel moment-to-moment probing of NREMS vulnerability and propose that chronic pain-induced sleep complaints arise from perturbed arousability.

【 授权许可】

CC BY