Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development

eLife

Joachim Wittbrodt¹  Lucie Zilova¹  Venera Weinhardt¹  Tinatini Tavhelidse¹  Thomas Thumberger¹  Christina Schlagheck² 

[1] Centre for Organismal Studies Heidelberg, Heidelberg University, Heidelberg, Germany;Centre for Organismal Studies Heidelberg, Heidelberg University, Heidelberg, Germany;Heidelberg International Biosciences Graduate School HBIGS and HeiKa Graduate School on “Functional Materials”, Heidelberg, Germany;

关键词: medaka;    organoids;    retina;    in vitro organogenesis;    zebrafish;    Other;   

DOI  :  10.7554/eLife.66998

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Organoids derived from pluripotent stem cells promise the solution to current challenges in basic and biomedical research. Mammalian organoids are however limited by long developmental time, variable success, and lack of direct comparison to an in vivo reference. To overcome these limitations and address species-specific cellular organization, we derived organoids from rapidly developing teleosts. We demonstrate how primary embryonic pluripotent cells from medaka and zebrafish efficiently assemble into anterior neural structures, particularly retina. Within 4 days, blastula-stage cell aggregates reproducibly execute key steps of eye development: retinal specification, morphogenesis, and differentiation. The number of aggregated cells and genetic factors crucially impacted upon the concomitant morphological changes that were intriguingly reflecting the in vivo situation. High efficiency and rapid development of fish-derived organoids in combination with advanced genome editing techniques immediately allow addressing aspects of development and disease, and systematic probing of impact of the physical environment on morphogenesis and differentiation.

【 授权许可】

CC BY