Influence of the densities and nutritional components of bacterial colonies on the culture-enriched gut bacterial community structure

AMB Express

Jieyu Liu¹  Shaojun Di¹  Minna Wu¹  Fan Yang¹  Mengjie Yang¹  Min Li¹  Puze Li¹  Jingjing Wang¹  Yahan Chang¹  Dong Yan¹  Shijun Ni¹  Yanrong Gu² 

[1] Xinxiang Key Laboratory of Pathogenic Biology, Department of Microbiology, School of Basic Medical Sciences, Xinxiang Medical University, 601 Jinsui Road, Hongqi District, 453003, Xinxiang, Henan, China;Xinxiang Key Laboratory of Pathogenic Biology, Department of Microbiology, School of Basic Medical Sciences, Xinxiang Medical University, 601 Jinsui Road, Hongqi District, 453003, Xinxiang, Henan, China;Laboratory of Genetic Regulators in the Immune System, Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine, School of Laboratory Medicine, Xinxiang Medical University, 453003, Xinxiang, Henan, China;

关键词: Culture-enriched;    Gut bacterial community;    Culture methods;    Diversity;    Nutritional components of media;    Density;   

DOI  :  10.1186/s13568-021-01240-6

来源: Springer

【 摘 要 】

Isolating relevant microorganisms is still a substantial challenge that limits the use of bacteria in the maintenance of human health. To confirm which media and which bacterial colony densities can enrich certain kinds of bacteria, we selected eight common media and used them to enrich the gut microorganisms on agar plates. Then, we calculated the numbers of bacterial colonies and collected the bacterial culture mixtures from each kind of medium. Using the Illumina HiSeq platform, we analyzed the composition and diversity of the culture-enriched gut bacterial community. Our data suggested that medium supplemented with blood could increase the diversity of the bacterial community. In addition, beef powder and peptone could significantly change the culture-enriched bacterial community. A moderate density (100–150 colony-forming units per plate) was optimal for obtaining the highest diversity on the agar. Similarly, membrane transport was significantly enriched in the moderate-density group, which indicated a more active metabolism in this density range. Overall, these results reveal the optimal culture conditions, including the densities of colonies and nutritional components for various gut bacteria, that provide a novel strategy for isolating bacteria in a way that is targeted and avoids blinded and repetitive work.

【 授权许可】

CC BY