Differential expression of long non-coding RNA Regulator of reprogramming and its molecular mechanisms in polycystic ovary syndrome

Journal of Ovarian Research

Zhihong Zhang¹  Jing Shao¹  Yunjiang Cai²  Min Sang³  Siqin Liu⁴ 

[1] Department of Obstetrics and Gynecology, General Hospital of Daqing Oilfield, 163000, Daqing, Heilongjiang, China;Department of Psychology, Harbin Medical Univercity (Daqing), 163319, Daqing, Heilongjiang, China;Gynecology Clinic, The First Hospital of Harbin, No.151 Diduan Street, 150010, Harbin, Heilongjiang, China;Laboratory Department, General Hospital of Daqing Oilfield, 163000, Daqing, Heilongjiang, China;

关键词: Polycystic ovary syndrome;    MiR-206;    Regulator of reprogramming;    Vascular endothelial growth factor;   

DOI  :  10.1186/s13048-021-00829-6

来源: Springer

【 摘 要 】

BackgroundPolycystic ovary syndrome (PCOS) is a common endocrine disease in women of reproductive age. Multiple studies have shown that long non-coding RNAs (lncRNA) and microRNAs (miRNA) play a role in PCOS. This study aimed to explore the role and molecular mechanism of lncRNA -Regulator of reprogramming (lncROR) in PCOS.ResultsExpression level of lncROR in PCOS patients was up-regulated, while level of miR-206 was down-regulated in comparison with control group (P < 0.001). Logistics regression analysis showed that lncROR and miR-206 were independent predictors of PCOS. The ROC curve showed that lncROR had a high diagnostic value for PCOS with an AUC value of 0.893. Pearson correlation coefficient indicated that the expression level of miR-206 was negatively correlated with the level of lncROR. CCK-8 assay and apoptosis assay revealed that downregulation of lncROR up-regulated the expression of miR-206, thereby inhibiting cell proliferation and promoting cell apoptosis. However, silencing the expression of miR-206 reversed the above effects caused by down-regulation of lncROR expression. Luciferase reporter gene assay suggested that there was a target relationship between lncROR and miR-206. VEGF was proved to be the target gene of miR-206.ConclusionsHighly expressed lncROR indirectly up-regulated the expression of VEGF by down-regulating the expression of miR-206, thereby promoting the proliferation of KGN cells and inhibiting apoptosis, and further promoting the development of PCOS.

【 授权许可】

CC BY