A synbiotic intervention modulates meta-omics signatures of gut redox potential and acidity in elective caesarean born infants

BMC Microbiology

Xuezhi Bi¹  Shuwen Chen¹  Ying Swan Ho¹  Yee Jiun Kok¹  Shi Ya Mak¹  Enzo Acerbi²  Christophe Lay²  Rocio Martin³  Guus Roeselers³  Jan Knol⁴  Collins Wenhan Chu⁵  Song Jie⁵  Eliza Ho⁵  Paola Florez de Sessions⁵  Martin L. Hibberd⁶  Rajeshwar Rao⁷  Mei Chien Chua⁷  Wen Chin Chiang⁷  Anne E. N. Goh⁷  Nipon Khemapech⁸  Surasith Chaithongwongwatthana⁸  Voranush Chongsrisawat⁸  Daniela I. Drautz-Moses⁹  Rikky Wenang Purbojati⁹  Stephan C. Schuster⁹ 

[1] Bioprocessing Technology Institute, Singapore, Singapore;Danone Nutricia Research, Singapore, Singapore;Danone Nutricia Research, Utrecht, The Netherlands;Danone Nutricia Research, Utrecht, The Netherlands;Wageningen University, Wageningen, The Netherlands;Genome Institute of Singapore, Singapore, Singapore;Genome Institute of Singapore, Singapore, Singapore;London School of Hygiene and Tropical Medicine, London, UK;KK Women’s and Children’s Hospital, Singapore, Singapore;King Chulalongkorn Memorial Hospital, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand;Singapore Centre For Environmental Life Sciences Engineering (SCELSE), Nanyang Technological University, Singapore, Singapore;

关键词: Microbiome;    Infant;    C-section;    Synbiotics;   

DOI  :  10.1186/s12866-021-02230-1

来源: Springer

【 摘 要 】

BackgroundThe compromised gut microbiome that results from C-section birth has been hypothesized as a risk factor for the development of non-communicable diseases (NCD). In a double-blind randomized controlled study, 153 infants born by elective C-section received an infant formula supplemented with either synbiotic, prebiotics, or unsupplemented from birth until 4 months old. Vaginally born infants were included as a reference group. Stool samples were collected from day 3 till week 22. Multi-omics were deployed to investigate the impact of mode of delivery and nutrition on the development of the infant gut microbiome, and uncover putative biological mechanisms underlying the role of a compromised microbiome as a risk factor for NCD.ResultsAs early as day 3, infants born vaginally presented a hypoxic and acidic gut environment characterized by an enrichment of strict anaerobes (Bifidobacteriaceae). Infants born by C-section presented the hallmark of a compromised microbiome driven by an enrichment of Enterobacteriaceae. This was associated with meta-omics signatures characteristic of a microbiome adapted to a more oxygen-rich gut environment, enriched with genes associated with reactive oxygen species metabolism and lipopolysaccharide biosynthesis, and depleted in genes involved in the metabolism of milk carbohydrates. The synbiotic formula modulated expression of microbial genes involved in (oligo)saccharide metabolism, which emulates the eco-physiological gut environment observed in vaginally born infants. The resulting hypoxic and acidic milieu prevented the establishment of a compromised microbiome.ConclusionsThis study deciphers the putative functional hallmarks of a compromised microbiome acquired during C-section birth, and the impact of nutrition that may counteract disturbed microbiome development.Trial registrationThe study was registered in the Dutch Trial Register (Number: 2838) on 4th April 2011.

【 授权许可】

CC BY