Comparative anatomical and transcriptomic insights into Vaccinium corymbosum flower bud and fruit throughout development

BMC Plant Biology

Lei Yu¹  Kailing Cai¹  Zhuoyi Wang¹  Liangmiao Liu¹  Li Yang²  Weidong Guo²  Yongqaing Li²  Yu Zong²  Fanglei Liao²  Manman Chen³ 

[1] College of Chemistry and Life Sciences, Zhejiang Normal University, 321004, Jinhua, Zhejiang, P. R. China;College of Chemistry and Life Sciences, Zhejiang Normal University, 321004, Jinhua, Zhejiang, P. R. China;Zhejiang Provincial Key Laboratory of Biotechnology on Specialty Economic Plants, Zhejiang Normal University, 321004, Jinhua, Zhejiang, P. R. China;Zhejiang College of Security Technology, 325000, Wenzhou, Zhejiang, P. R. China;

关键词: Cell proliferation;    Cell expansion;    Fruit development;    Comparative transcriptome;    TIFY;    Blueberry;   

DOI  :  10.1186/s12870-021-03067-6

来源: Springer

【 摘 要 】

BackgroundBlueberry (Vaccinium spp.) is characterized by the production of berries that are smaller than most common fruits, and the underlying mechanisms of fruit size in blueberry remain elusive. V. corymbosum ‘O’Neal’ and ‘Bluerain’ are commercial southern highbush blueberry cultivars with large- and small-size fruits, respectively, which mature ‘O’Neal’ fruits are 1 ~ 2-fold heavier than those of ‘Bluerain’. In this study, the ontogenetical patterns of ‘O’Neal’ and ‘Bluerain’ hypanthia and fruits were compared, and comparative transcriptomic analysis was performed during early fruit development.ResultsV. corymbosum ‘O’Neal’ and ‘Bluerain’ hypanthia and fruits exhibited intricate temporal and spatial cell proliferation and expansion patterns. Cell division before anthesis and cell expansion after fertilization were the major restricting factors, and outer mesocarp was the key tissue affecting fruit size variation among blueberry genotypes. Comparative transcriptomic and annotation analysis of differentially expressed genes revealed that the plant hormone signal transduction pathway was enriched, and that jasmonate-related TIFYs genes might be the key components orchestrating other phytohormones and influencing fruit size during early blueberry fruit development.ConclusionsThese results provided detailed ontogenetic evidence for determining blueberry fruit size, and revealed the important roles of phytohormone signal transductions involving in early fruit development. The TIFY genes could be useful as markers for large-size fruit selection in the current breeding programs of blueberry.

【 授权许可】

CC BY