Anti-angiogenesis and anti-metastasis effects of Polyphyllin VII on Hepatocellular carcinoma cells in vitro and in vivo

Chinese Medicine

Yi Zhang¹  Qingrui Li²  Chao Zhang³  Kechun Liu⁴  Rongchun Wang⁴  Liwen Han⁵  Shudan Wang⁶  Chaoying Li⁶  Chengwei He⁷  Haixia Chen⁸  Guozheng Qin⁹ 

[1] Beijing University of Chinese Medicine, 100102, Beijing, China;Beijing University of Chinese Medicine, 100102, Beijing, China;Aerospace Central Hospital, 100049, Beijing, China;Beijing University of Chinese Medicine, 100102, Beijing, China;State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, 999078, Macao, China;Biology Institute, Qilu University of Technology (Shandong Academy of Sciences), 250103, Jinan, China;Institute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, 250062, Jinan, China;School of Pharmaceutical Sciences, Changchun University of Chinese Medicine, 130117, Changchun, China;State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, 999078, Macao, China;Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, 300072, Tianjin, China;Yunnan Provincial Hospital of Traditional Chinese Medicine/The First Affiliated Hospital of Yunnan University of Chinese Medicine, 650021, Kunming, China;

关键词: Polyphyllin VII;    Hepatocellular carcinoma cells;    Anti-angiogenesis;    Anti-metastasis;    NF-κB/MMP-9/VEGF pathway;   

DOI  :  10.1186/s13020-021-00447-w

来源: Springer

【 摘 要 】

BackgroundPolyphyllin VII (PP7), a steroidal saponin from P. polyphylla has been found to exert strong anticancer activity. Little is known about the anti-angiogenesis and anti-metastasis properties of PP7. In this study, the anti-angiogenic and anti-metastatic effects of PP7 on HCC and the molecular mechanisms were evaluated.MethodsEffect of PP7 on angiogenesis was assessed by tube formation assay and applied a transgenic Tg(fli1:EGFP) zebrafish model. Effects of PP7 on tumor metastasis and invasion were examined in cell migration and invasion assay, zebrafish tumor xenograft models and lung metastasis mouse models. The protein levels were examined by Western blotting.ResultsPP7 significantly decreased the tube formation of human umbilical vein endothelial cells, the number and length of ISVs and SIVs of transgenic zebrafish, and the metastasis and invasion of cancer cells in vitro and in vivo. The anti-angiogenic and anti-metastatic effects of PP7 in HepG2 cells were attributable, at least partially, to downregulated NF-κB/MMP-9/VEGF signaling pathway.ConclusionThis study demonstrates that PP7 possesses strong anti-angiogenesis and anti-metastasis activities, suggesting that PP7 could be a potential candidate agent for HCC treatment.

【 授权许可】

CC BY