Occurrence and potentially zoonotic genotypes of Enterocytozoon bieneusi in wild rhesus macaques (Macaca mulatta) living in Nanwan Monkey Island, Hainan, China: a public health concern

BMC Veterinary Research

Hui-Cong Huang¹  Feng Tan¹  Wei Zhao²  Guang-Xu Ren³  Yu Qiang³  Gang Lu³  Huan-Huan Zhou⁴ 

[1] Department of Parasitology, Wenzhou Medical University, 325035, Wenzhou, Zhejiang, China;Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, 571199, Haikou, China;Department of Parasitology, Wenzhou Medical University, 325035, Wenzhou, Zhejiang, China;Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, 571199, Haikou, China;Department of Pathogenic Biology, Hainan Medical University, Haikou, Hainan, China;Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, Hainan, China;Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, 571199, Haikou, China;Qingdao Shinan District Center for Disease Control and Prevention, 266071, Qingdao, Shandong, China;

关键词: Enterocytozoon bieneusi;    Rhesus macaques;    Hainan;    Zoonotic;   

DOI  :  10.1186/s12917-021-02916-8

来源: Springer

【 摘 要 】

BackgroundEnterocytozoon bieneusi, a microsporidian species, is a zoonotic pathogen found in both humans and animals. Here, we determined the prevalence, explored the different genotypes of E. bieneusi in wild rhesus macaques (Macaca mulatta) (Hainan Island of China), and assessed their zoonotic potential.MethodsWe collected 173 fecal specimens from wild rhesus macaques living in Nanwan Monkey Island, Hainan, China. Subsequently, we identified and genotyped E. bieneusi using nested PCR analysis amplification of the internal transcribed spacer region (ITS) of the rRNA gene. Lastly, a neighbor-joining tree was built based on gene sequences from the ITS region of E. bieneusi.ResultsOf the 173 specimens from wild rhesus macaques, 26 (15%) were infected with E. bieneusi. We identified six genotypes of E. bieneusi, of which five were known: PigEBITS7 (n = 20), D (n = 2), Type IV (n = 1), Peru6 (n = 1), Henan-III (n = 1), and a novel genotype: HNM-IX (n = 1). From the phylogenetic analysis, the six genotypes identified here were all clustered into zoonotic group 1.ConclusionThis study is the first report to detect E. bieneusi infection in wild rhesus macaques from Hainan, China. Human-pathogenic genotypes D, Henan-III, Peru6, PigEbITS7, and Type IV in the wild rhesus macaques support these animals infected with E. bieneusi have a public health significance.

【 授权许可】

CC BY