Transcriptome analysis reveals Vernalization is independent of cold acclimation in Arabidopsis

BMC Genomics

Qian Hu¹  Jia Fu Jiang¹  Fei Li¹  Fadi Chen¹ 

[1] State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China;

关键词: Arabidopsis;    Transcriptome profiling;    Cold stress;    Vernalization;    FLC;   

DOI  :  10.1186/s12864-021-07763-3

来源: Springer

【 摘 要 】

BackgroundThrough vernalization, plants achieve flowering competence by sensing prolonged cold exposure (constant exposure approximately 2-5 °C). During this process, plants initiate defense responses to endure cold conditions. Here, we conducted transcriptome analysis of Arabidopsis plants subjected to prolonged cold exposure (6 weeks) to explore the physiological dynamics of vernalization and uncover the relationship between vernalization and cold stress.ResultsTime-lag initiation of the two pathways and weighted gene co-expression network analysis (WGCNA) revealed that vernalization is independent of cold acclimation. Moreover, WGCNA revealed three major networks involving ethylene and jasmonic acid response, cold acclimation, and chromatin modification in response to prolonged cold exposure. Finally, throughout vernalization, the cold stress response is regulated via an alternative splicing-mediated mechanism.ConclusionThese findings illustrate a comprehensive picture of cold stress- and vernalization-mediated global changes in Arabidopsis.

【 授权许可】

CC BY