Molecular typing, biofilm production, and detection of carbapenemase genes in multidrug-resistant Acinetobacter baumannii isolated from different infection sites using ERIC-PCR in Hamadan, west of Iran

BMC Pharmacology and Toxicology

Leili Shokoohizadeh¹  Pezhman Karami¹  Mohammad Taheri¹  Mahsa Movahedi²  Omid Zarei²  Maryam Hazhirkamal² 

[1] Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran;Student Research Committee, Hamadan University of Medical Sciences, Hamadan, Iran;

关键词: Molecular typing;    Biofilm formation;    MBL;    MDR;    Acinetobacter baumannii;   

DOI  :  10.1186/s40360-021-00504-y

来源: Springer

【 摘 要 】

BackgroundAcinetobacter baumannii is an opportunistic pathogen that can cause several kinds of nosocomial infections. Increasing antibiotic resistance as well as identifying genetic diversity and factors associated with pathogenicity and prevalence of this bacterium is important. The aim of this study was the investigation of molecular typing, biofilm production, and detection of carbapenemase genes in multidrug-resistant Acinetobacter baumannii isolated from different infection sites using ERIC-PCR in Iran.MethodsForty isolates of A. baumannii were obtained from various wards of the central hospital, in the west of Iran. Phenotypic identification and genetic diversity, biofilm production assay, and detection of Carbapenemase genes carried out.ResultsTracheal samples 26 (61.9 %) are the most frequent isolates, and 95 % of isolates were identified as MDR. 32.5 % of all A. baumannii strains were capable to form a strong biofilm. It was founded that antimicrobial resistance patterns had a significant relationship with strong biofilm formation (P = 0.001). Most frequencies of the studied genes were in the order of VIM (81 %), SPM (45.2 %), and IMP (35.7 %) genes. The VIM gene was the most frequent in all isolates which were significant (P = 0.006). 14 different ERIC-types were observed including 7 common types and 7 unique or single types. F type is the largest common type consisting of nine isolates and B, D, and E types contain two isolates separately.ConclusionsERIC-PCR technique was used to genetically classify A. baumannii isolates as one of the most common microorganisms in nosocomial infections.

【 授权许可】

CC BY