Downregulation of exosomal let-7d and miR-16 in idiopathic pulmonary fibrosis

BMC Pulmonary Medicine

Lucia Catucci¹  Roberto Rana²  Massimo Conese²  Filomena Simone²  Sante Di Gioia²  Carla M. I. Quarato²  Maria P. Foschino-Barbaro³  Piera Soccio³  Giulia Scioscia³  Donato Lacedonia³  Francesco Sollitto⁴  Grazia P. Palladino⁵ 

[1] Department of Chemistry, University “Aldo Moro” of Bari, 70126, Bari, Italy;Department of Medical and Surgical Sciences, University of Foggia, 71122, Foggia, Italy;Department of Medical and Surgical Sciences, University of Foggia, 71122, Foggia, Italy;Institute of Respiratory Diseases, Policlinico Riuniti of Foggia, 71122, Foggia, Italy;Institute of Thoracic Surgery, University of Foggia, 71122, Foggia, Italy;Medical Genetics, Department of Laboratory Diagnostics, Policlinico Riuniti of Foggia, 71122, Foggia, Italy;

关键词: Idiopathic pulmonary fibrosis;    microRNA;    Exosomes;    Biomarkers;   

DOI  :  10.1186/s12890-021-01550-2

来源: Springer

【 摘 要 】

BackgroundIdiopathic Pulmonary Fibrosis (IPF) is a degenerative interstitial lung disease with both a poor prognosis and quality of life once the diagnosis is made. In the last decade many features of the disease have been investigated to better understand the pathological steps that lead to the onset of the disease and, moreover, different types of biomarkers have been tested to find valid diagnostic, prognostic and therapy response predictive ones. In the complexity of IPF, microRNA (miRNAs) biomarker investigation seems to be promising.MethodsWe analysed the expression of five exosomal miRNAs supposed to have a role in the pathogenesis of the disease from serum of a group of IPF patients (n = 61) and we compared it with the expression of the same miRNAs in a group of healthy controls (n = 15).ResultsIn the current study what emerged is let-7d down-regulation and, unexpectedly, miR-16 significant down-regulation. Moreover, through a cross-sectional analysis, a clustering of the expression of miR-16, miR-21 and miR-26a was found.ConclusionsThese findings could help the individuation of previously unknown key players in the pathophysiology of IPF and, most interestingly, more specific targets for the development of effective medications.

【 授权许可】

CC BY