High-throughput surface marker screen on primary human breast tissues reveals further cellular heterogeneity

Breast Cancer Research

John Stingl¹  Siru Virtanen¹  Carlos Caldas²  Mona Shehata³  Reiner Schulte⁴ 

[1] CRUK Cambridge Institute, University of Cambridge, CB2 0RE, Cambridge, UK;CRUK Cambridge Institute, University of Cambridge, CB2 0RE, Cambridge, UK;Cambridge Breast Unit, Addenbrookes Hospital, Cambridge University Hospital NHS Foundation Trust and NIHR Cambridge Biomedical Research Centre, Cambridge, UK;CRUK Cambridge Institute, University of Cambridge, CB2 0RE, Cambridge, UK;Medical Research Council Cancer Unit, University of Cambridge, Hills Road, CB2 0XZ, Cambridge, UK;Cambridge Institute for Medical Research, Cambridge University, CB2 0XY, Cambridge, UK;

关键词: Normal breast;    Surface markers;    Breast epithelial cells;    Stromal;    Luminal progenitor;    Antibody screen;   

DOI  :  10.1186/s13058-021-01444-5

来源: Springer

【 摘 要 】

BackgroundNormal human breast tissues are a heterogeneous mix of epithelial and stromal subtypes in different cell states. Delineating the spectrum of cellular heterogeneity will provide new insights into normal cellular properties within the breast tissue that might become dysregulated in the initial stages of cancer. Investigation of surface marker expression provides a valuable approach to resolve complex cell populations. However, the majority of cell surface maker expression of primary breast cells have not been investigated.MethodsTo determine the differences in expression of a range of uninvestigated cell surface markers between the normal breast cell subpopulations, primary human breast cells were analysed using high-throughput flow cytometry for the expression of 242 cell surface proteins in conjunction with EpCAM/CD49f staining.ResultsWe identified 35 surface marker proteins expressed on normal breast epithelial and/or stromal subpopulations that were previously unreported. We also show multiple markers were equally expressed in all cell populations (e.g. CD9, CD59, CD164) while other surface markers were confirmed to be enriched in different cell lineages: CD24, CD227 and CD340 in the luminal compartment, CD10 and CD90 in the basal population, and CD34 and CD140b on stromal cells.ConclusionsOur dataset of CD marker expression in the normal breast provides better definition for breast cellular heterogeneity.

【 授权许可】

CC BY