Study on the differences of gene expression between pear and apple wild cultivation materials based on RNA-seq technique

BMC Plant Biology

Chao Yue¹  Shaoling Zhang²  Min Kong²  Meng Li²  Juyou Wu²  Huangwei Zhang³  Yuyan Zhang⁴  Jim M. Dunwell⁵ 

[1] China Tobacco Jiangsu Industrial Co., Ltd, 210019, Nanjing, China;College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China;College of Horticulture, Nanjing Agricultural University, 210095, Nanjing, China;College of Agro-Grassland Science, Nanjing Agricultural University, 210095, Nanjing, China;Institute of Pomology, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory of Horticultural Crop Genetic Improvement, 210014, Nanjing, Jiangsu, China;School of Agriculture, Policy and Development, University of Reading, Earley Gate, Reading, UK;

关键词: Pear;    Apple;    Wild cultivar;    Differential expression;    Domestication;    RNA-seq;   

DOI  :  10.1186/s12870-021-03051-0

来源: Springer

【 摘 要 】

BackgroundPears and apples are both perennial deciduous trees of the Rosaceae family, and both are important economic fruit trees worldwide. The emergence of many varieties in the market has been mostly domesticated from wild to cultivated and regulated by the differential expression of genes. However, the molecular process and pathways underlying this phenomenon remain unclear. Four typical wild and cultivar pear and apple trees at three developmental stages were used in our study to investigate the molecular process at the transcriptome level.ResultPhysiological observations indicated the obvious differences of size, weight, sugar acid content and peel color in wild and cultivar fruit among each developmental stage. Using next-generation sequencing based RNA-seq expression profiling technology, we produced a transcriptome in procession of a large fraction of annotated pear and apple genes, and provided a molecular basis underlying the phenomenon of wild and cultivar fruit tree differences. 5921 and 5744 differential expression genes were identified in pear and apple at three developmental stages respectively. We performed temporal and spatial differential gene expression profiling in developing fruits. Several key pathways such as signal transduction, photosynthesis, translation and many metabolisms were identified as involved in the differentiation of wild and cultivar fruits.ConclusionIn this study, we reported on the next-generation sequencing study of the temporal and spatial mRNA expression profiling of pear and apple fruit trees. Also, we demonstrated that the integrated analysis of pear and apple transcriptome, which strongly revealed the consistent process of domestication in Rosaceae fruit trees. The results will be great influence to the improvement of cultivar species and the utilization of wild resources.

【 授权许可】

CC BY