Near-Single-Cell Proteomics Profiling of the Proximal Tubular and Glomerulus of the Normal Human Kidney

Frontiers in Medicine

Yingbao Yang¹  Jinghui Luo¹  Tara K. Sigdel²  Sudeshna Roy²  Priyanka Rashmi²  Izabella Damm²  Swastika Sur²  Minnie M. Sarwal²  Juliane Liberto²  Andrew Schroeder²  Rui Zhao³  Ying Zhu³  Paul D. Piehowski⁴  Joshua R. Hansen⁴  Wei-Jun Qian⁴  Adam C. Swensen⁴ 

[1] Department of Pathology, University of Michigan, Ann Arbor, MI, United States;Division of MultiOrgan Transplantation, Department of Surgery, University of California, San Francisco, San Francisco, CA, United States;Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, United States;Pacific Northwest National Laboratory, Biological Sciences Division, Richland, WA, United States;

关键词: glomerulus;    mass spectrometry;    single cell analysis;    proteomics;    kidney;   

DOI  :  10.3389/fmed.2020.00499

来源: Frontiers

【 摘 要 】

Molecular assessments at the single cell level can accelerate biological research by providing detailed assessments of cellular organization and tissue heterogeneity in both disease and health. The human kidney has complex multi-cellular states with varying functionality, much of which can now be completely harnessed with recent technological advances in tissue proteomics at a near single-cell level. We discuss the foundational steps in the first application of this mass spectrometry (MS) based proteomics method for analysis of sub-sections of the normal human kidney, as part of the Kidney Precision Medicine Project (KPMP). Using ~30–40 laser captured micro-dissected kidney cells, we identified more than 2,500 human proteins, with specificity to the proximal tubular (PT; n = 25 proteins) and glomerular (Glom; n = 67 proteins) regions of the kidney and their unique metabolic functions. This pilot study provides the roadmap for application of our near-single-cell proteomics workflow for analysis of other renal micro-compartments, on a larger scale, to unravel perturbations of renal sub-cellular function in the normal kidney as well as different etiologies of acute and chronic kidney disease.

【 授权许可】

CC BY