Chromatographic and electrophoretic methods for nanodisc purification and analysis

Reviews in analytical chemistry

article

Bo Højen Justesen¹  Thomas Günther-Pomorski¹ 

[1] Department of Plant and Environmental Sciences, University of Copenhagen

关键词: chromatography;    free flow electrophoresis;    mass spectrometry;    nanodiscs;    native PAGE;   

DOI  :  10.1515/revac-2014-0014

学科分类：社会科学、人文和艺术（综合）

来源: De Gruyter

【 摘 要 】

Soluble nanoscale lipid bilayers, termed nanodiscs, are widely used in science for studying the membrane-anchored and integral membrane protein complexes under defined experimental conditions. Although their formation occurs by a self-assembly process, nanodisc purification and the verification of proper reconstitution are still major challenges during the sample preparation. This review gives an overview of the methods used for purifying and analyzing nanodiscs and nanodisc-reconstituted membrane proteins, with an emphasis on the chromatographic and electrophoretic approaches.

【 授权许可】

CC BY