Nanodisc characterization by analytical ultracentrifugation

Nanotechnology Reviews

article

Sayaka Inagaki¹  Rodolfo Ghirlando² 

[1] Basic Neurosciences Program, National Institutes of Neurological Disorders and Stroke, National Institutes of Health;Laboratory of Molecular Biology, National Institutes of Diabetes and Digestive and Kidney Diseases, National Institutes of Health

关键词: analytical ultracentrifugation;    lipid;    membrane protein;    nanodisc;   

DOI  :  10.1515/ntrev-2016-0082

学科分类：社会科学、人文和艺术（综合）

来源: De Gruyter

【 摘 要 】

Due to their unique properties, tunable size, and ability to provide a near native lipid environment, nanodiscs have found widespread use for the structural and functional studies of reconstituted membrane proteins. They have also been developed, albeit in a few applications, for therapeutic and biomedical use. For these studies and applications, it is essential to characterize the nanodisc preparations in terms of their monodispersity, size, and composition, as these can influence the properties of the membrane protein of interest. Of the many biophysical methods utilized for the study and characterization of nanodiscs, we show that analytical ultracentrifugation is able to report on sample homogeneity, shape, size, composition, and membrane protein stoichiometry or oligomerization state in a direct and simple fashion. The method is truly versatile and does not require nanodisc modification or disassembly.

【 授权许可】

CC BY