Angular modulation of single-molecule fluorescence by gold nanoparticles on DNA origami templates

Nanophotonics

article

Friederike M. Möller¹  Phil Holzmeister¹  Tapasi Sen¹  Guillermo P. Acuna¹  Philip Tinnefeld¹ 

[1] NanoBioSciences Group, Institute for Physical and Theoretical Chemistry

关键词: DNA self-assembly;    fluorescence modulation;    nanoparticles;    nanophotonics;    single-molecule studies;   

DOI  :  10.1515/nanoph-2013-0011

学科分类：社会科学、人文和艺术（综合）

来源: De Gruyter

【 摘 要 】

We study the angular fluorescence intensity modulation of a single dye positioned near a spherical gold nanoparticle, induced by rotation of linearly polarized excitation light. Accurate positioning and alignment of nanoparticle and fluorophore with respect to each other and the incoming electric field is achieved by a three-dimensional, self-assembled DNA origami. An intensity map is obtained for a fixed distance and two different nanoparticle diameters, revealing polarization-dependent enhancement and quenching of fluorescence intensity in good agreement to numerical simulations.

【 授权许可】

CC BY