Nanophotonics | |
Localization STED (LocSTED) microscopy with 15 nm resolution | |
article | |
Sujitha Puthukodan1  Eljesa Murtezi1  Jaroslaw Jacak3  Thomas A. Klar1  | |
[1] Institute of Applied Physics and Linz Institute of Technology (LIT), Johannes Kepler University;Department of Polymer Science and Technology, Istanbul Technical University;University of Applied Sciences Upper Austria | |
关键词: stimulated emission depletion (STED) microscopy; single-molecule localization microscopy (SMLM); protein nanoanchors; single-molecule imaging; | |
DOI : 10.1515/nanoph-2019-0398 | |
学科分类:社会科学、人文和艺术(综合) | |
来源: De Gruyter | |
【 摘 要 】
We present localization with stimulated emission depletion (LocSTED) microscopy, a combination of STED and single-molecule localization microscopy (SMLM). We use the simplest form of a STED microscope that is cost effective and synchronization free, comprising continuous wave (CW) lasers for both excitation and depletion. By utilizing the reversible blinking of fluorophores, single molecules of Alexa 555 are localized down to ~5 nm. Imaging fluorescently labeled proteins attached to nanoanchors structured by STED lithography shows that LocSTED microscopy can resolve molecules with a resolution of at least 15 nm, substantially improving the classical resolution of a CW STED microscope of about 60 nm. LocSTED microscopy also allows estimating the total number of proteins attached on a single nanoanchor.
【 授权许可】
CC BY
【 预 览 】
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RO202107200003442ZK.pdf | 1411KB | download |