Three-dimensional spatiotemporal tracking of nano-objects diffusing in water-filled optofluidic microstructured fiber

Nanophotonics

article

Shiqi Jiang¹  Ronny Förster¹  Malte Plidschun¹  Jens Kobelke¹  Ron Fatobene Ando¹  Markus A. Schmidt¹ 

[1] Leibniz Institute of Photonic Technology;Otto Schott Institute of Materials Research (OSIM), Friedrich Schiller University Jena;Abbe Center of Photonics and Faculty of Physics, Friedrich-Schiller-University Jena

关键词: biosensing;    confined diffusion;    elastic light scattering;    nanofluidics;    resistance coefficient;    3D tracking of single nanoparticle;   

DOI  :  10.1515/nanoph-2020-0330

学科分类：社会科学、人文和艺术（综合）

来源: De Gruyter

【 摘 要 】

Three-dimensional (3D) tracking of nano-objects represents a novel pathway for understanding dynamic nanoscale processes within bioanalytics and life science. Here we demonstrate 3D tracking of diffusing 100 nm gold nanosphere within a water-filled optofluidic fiber via elastic light scattering–based position retrieval. Specifically, the correlation between intensity and position inside a region of a fiber-integrated microchannel has been used to decode the axial position from the scattered intensity, while image processing–based tracking was used in the image plane. The 3D trajectory of a diffusing gold nanosphere has been experimentally determined, while the determined diameter analysis matches expectations. Beside key advantages such as homogenous light-line illumination, low-background scattering, long observation time, large number of frames, high temporal and spatial resolution and compatibility with standard microscope, the particular properties of operating with water defines a new bioanalytical platform that is highly relevant for medical and life science applications.

【 授权许可】

CC BY