Nanophotonics | |
Three-dimensional spatiotemporal tracking of nano-objects diffusing in water-filled optofluidic microstructured fiber | |
article | |
Shiqi Jiang1  Ronny Förster1  Malte Plidschun1  Jens Kobelke1  Ron Fatobene Ando1  Markus A. Schmidt1  | |
[1] Leibniz Institute of Photonic Technology;Otto Schott Institute of Materials Research (OSIM), Friedrich Schiller University Jena;Abbe Center of Photonics and Faculty of Physics, Friedrich-Schiller-University Jena | |
关键词: biosensing; confined diffusion; elastic light scattering; nanofluidics; resistance coefficient; 3D tracking of single nanoparticle; | |
DOI : 10.1515/nanoph-2020-0330 | |
学科分类:社会科学、人文和艺术(综合) | |
来源: De Gruyter | |
【 摘 要 】
Three-dimensional (3D) tracking of nano-objects represents a novel pathway for understanding dynamic nanoscale processes within bioanalytics and life science. Here we demonstrate 3D tracking of diffusing 100 nm gold nanosphere within a water-filled optofluidic fiber via elastic light scattering–based position retrieval. Specifically, the correlation between intensity and position inside a region of a fiber-integrated microchannel has been used to decode the axial position from the scattered intensity, while image processing–based tracking was used in the image plane. The 3D trajectory of a diffusing gold nanosphere has been experimentally determined, while the determined diameter analysis matches expectations. Beside key advantages such as homogenous light-line illumination, low-background scattering, long observation time, large number of frames, high temporal and spatial resolution and compatibility with standard microscope, the particular properties of operating with water defines a new bioanalytical platform that is highly relevant for medical and life science applications.
【 授权许可】
CC BY
【 预 览 】
Files | Size | Format | View |
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RO202107200003175ZK.pdf | 2162KB | download |