Green synthesis of silver nanoparticles from deoiled brown algal extract via Box-Behnken based design and their antimicrobial and sensing properties

Green Processing and Synthesis

article

Saravana Periaswamy Sivagnanam¹  Adane Tilahun Getachew¹  Jae Hyung Choi²  Yong Beom Park²  Hee Chul Woo²  Byung Soo Chun¹ 

[1] Department of Food Science and Technology, Pukyong National University;Department of Chemical Engineering, Pukyong National University

关键词: antimicrobial activity;    Box-Behnken design;    deoiled Saccharina japonica;    green synthesis;    response surface methodology;    silver nanoparticles;   

DOI  :  10.1515/gps-2016-0052

来源: De Gruyter

【 摘 要 】

The aim of this work was to acquire even and sphere-shaped silver nanoparticles (AgNPs) using statistical design of experiment. AgNPs were produced by green synthesis method using deoiled Saccharina japonica powder obtained after supercritical carbon dioxide (Sc-CO 2 ) extraction. Based on the Box-Behnken design, three variables influencing the size of AgNPs produced were identified as silver nitrate (AgNO 3 ) concentration, temperature, and reaction time. Optimum conditions were determined using response surface methodology for synthesis of AgNPs. We found that increasing reaction time at low concentration of AgNO 3 resulted in smaller particle size, and conversely increasing reaction time at high concentration of AgNO 3 resulted in bigger particles. The obtained AgNPs were characterized by transmission electron microscopy, energy dispersive X-ray, X-ray diffraction analysis, and ultraviolet-visible and Fourier transform infrared spectroscopy techniques for particle size, distribution, aggregation, and anisotropy. The optimum operating conditions are 1 m m of AgNO 3 , 40°C, and 45 min with the smallest AgNPs size being 14.77 nm. The optimized AgNPs showed good antimicrobial activity and excellent sensing behavior towards hydrogen peroxide. The polyphenols present in aqueous AgNPs were evaluated by high-pressure liquid chromatography, which revealed the existence of chlorogenic acid and rutin.

【 授权许可】

CC BY