Synergistic Improvement of Carbohydrate and Lignin Processability by Biomimicking Biomass Processing

Frontiers in Energy Research

Naijia Hao¹  Somnath Shinde¹  Arthur J. Ragauskas²  Michelle L. Olson³  Katy C. Kao³  Samarthya Bhagia⁴  Joshua S. Yuan⁵  Man Li⁵  Zhi-Hua Liu⁵  Qiang Li⁵  Shangxian Xie⁶ 

[1] Department of Chemical & Biomolecular Engineering, The University of Tennessee, Knoxville, Knoxville, TN, United States;Department of Chemical & Biomolecular Engineering, The University of Tennessee, Knoxville, Knoxville, TN, United States;Joint Institute of Biological Sciences, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States;Department of Forestry, Wildlife, and Fisheries, Center for Renewable Carbon, The University of Tennessee Institute of Agriculture, Knoxville, TN, United States;Department of Chemical Engineering, Texas A&M University, College Station, TX, United States;Joint Institute of Biological Sciences, Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, United States;Synthetic and Systems Biology Innovation Hub, Texas A&M University, College Station, TX, United States;Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, United States;Synthetic and Systems Biology Innovation Hub, Texas A&M University, College Station, TX, United States;Department of Plant Pathology and Microbiology, Texas A&M University, College Station, TX, United States;College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China;

关键词: lignocellulosic biomass;    lipid;    organosolv;    Fenton;    formic acid;    pretreatment;    biomimicking processing;   

DOI  :  10.3389/fenrg.2020.00194

来源: Frontiers

【 摘 要 】

The sustainability and economic feasibility of modern biorefinery depend on the efficient processing of both carbohydrate and lignin fractions for value-added products. By mimicking the biomass degradation process in white-rote fungi, a tailored two-step fractionation process was developed to maximize the sugar release from switchgrass biomass and to optimize the lignin processability for bioconversion. Biomimicking biomass processing using Formic Acid: Fenton: Organosolv (F2O) and achieved high processability for both carbohydrate and lignin. Specifically, switchgrass pretreated by the F2O process had 99.6% of the theoretical yield for glucose release. The fractionated lignin was also readily processable by fermentation via Rhodococcus opacus PD630 with a lipid yield of 1.16 g/L. Scanning electron microscope analysis confirmed the fragmentation of switchgrass fiber and the cell wall deconstruction by the F2O process. 2D-HSQC NMR further revealed the cleavage of aryl ether linkages (β-O-4) in lignin components. These results revealed the mechanisms for efficient sugar release and lignin bioconversion. The F2O process demonstrated effective mimicking of natural biomass utilization system and paved a new path for improving the lignin and carbohydrate processability in next generation lignocellulosic biorefinery.

【 授权许可】

CC BY