Cardiac regenerative potential of cardiosphere‐derived cells from adult dog hearts

Journal of Cellular and Molecular Medicine

Michael Taylor Hensley⁴  James de Andrade⁴  Bruce Keene⁵  Kathryn Meurs⁵  Junnan Tang⁴  Zegen Wang²  Thomas G. Caranasos¹  Jorge Piedrahita⁴  Tao-Sheng Li³ 

[1] Division of Cardiothoracic Surgery, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA;The Cyrus Tang Hematology Center, Soochow University, Suzhou, China;Department of Stem Cell Biology, Atomic Bomb Disease Institute, Nagasaki University, Nagasaki, Japan;Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA;Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA

关键词: cardiosphere‐derived cells;    dogs;    dilated cardiomyopathy;    stem cell therapy;   

DOI  :  10.1111/jcmm.12585

来源: Wiley

【 摘 要 】

Abstract

The regenerative potential of cardiosphere-derived cells (CDCs) for ischaemic heart disease has been demonstrated in mice, rats, pigs and a recently completed clinical trial. The regenerative potential of CDCs from dog hearts has yet to be tested. Here, we show that canine CDCs can be produced from adult dog hearts. These cells display similar phenotypes in comparison to previously studied CDCs derived from rodents and human beings. Canine CDCs can differentiate into cardiomyocytes, smooth muscle cells and endothelial cells in vitro. In addition, conditioned media from canine CDCs promote angiogenesis but inhibit cardiomyocyte death. In a doxorubicin-induced mouse model of dilated cardiomyopathy (DCM), intravenous infusion of canine CDCs improves cardiac function and decreases cardiac fibrosis. Histology revealed that injected canine CDCs engraft in the mouse heart and increase capillary density. Out study demonstrates the regenerative potential of canine CDCs in a mouse model of DCM.

【 授权许可】

CC BY   
© 2015 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

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