Bone‐forming capacity of adult human nasal chondrocytes

Journal of Cellular and Molecular Medicine

Benjamin E Pippenger¹  Manuela Ventura²  Karoliina Pelttari¹  Sandra Feliciano¹  Claude Jaquiery¹  Arnaud Scherberich¹  X Frank Walboomers²  Andrea Barbero¹ 

[1] Departments of Surgery and of Biomedicine, University Hospital Basel, University of Basel, Basel, Switzerland;Department of Biomaterials, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands

关键词: nasal chondrocytes;    craniofacial bone;    intramembranous ossification;    preclinical studies;    stromal cells;   

DOI  :  10.1111/jcmm.12526

来源: Wiley

【 摘 要 】

Abstract

Nasal chondrocytes (NC) derive from the same multipotent embryological segment that gives rise to the majority of the maxillofacial bone and have been reported to differentiate into osteoblast-like cells in vitro. In this study, we assessed the capacity of adult human NC, appropriately primed towards hypertrophic or osteoblastic differentiation, to form bone tissue in vivo. Hypertrophic induction of NC-based micromass pellets formed mineralized cartilaginous tissues rich in type X collagen, but upon implantation into subcutaneous pockets of nude mice remained avascular and reverted to stable hyaline-cartilage. In the same ectopic environment, NC embedded into ceramic scaffolds and primed with osteogenic medium only sporadically formed intramembranous bone tissue. A clonal study could not demonstrate that the low bone formation efficiency was related to a possibly small proportion of cells competent to become fully functional osteoblasts. We next tested whether the cues present in an orthotopic environment could induce a more efficient direct osteoblastic transformation of NC. Using a nude rat calvarial defect model, we demonstrated that (i) NC directly participated in frank bone formation and (ii) the efficiency of survival and bone formation by NC was significantly higher than that of reference osteogenic cells, namely bone marrow-derived mesenchymal stromal cells. This study provides a proof-of-principle that NC have the plasticity to convert into bone cells and thereby represent an easily available cell source to be further investigated for craniofacial bone regeneration.

【 授权许可】

CC BY   
© 2015 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.