Non‐invasive imaging of allogeneic transplanted skin graft by 131I‐anti‐TLR5 mAb

Journal of Cellular and Molecular Medicine

Hukui Sun¹  Guangjie Yang¹  Ting Liang¹  Chao Zhang¹  Jing Song¹  Jiankui Han¹ 

[1] Key Laboratory for Experimental Teratology of the Ministry of Education and Institute of Experimental Nuclear Medicine, School of Medicine, Shandong University, Ji'nan, Shandong, China

关键词: 131I‐anti‐TLR5 mAb;    18F‐FDG;    allo‐treated;    rapamycin;    phosphor‐autoradiography;   

DOI  :  10.1111/jcmm.12423

来源: Wiley

【 摘 要 】

Abstract

Although ¹⁸F-fluorodeoxyglucose (¹⁸F-FDG) uptake can be used for the non-invasive detection and monitoring of allograft rejection by activated leucocytes, this non-specific accumulation is easily impaired by immunosuppressants. Our aim was to evaluate a ¹³¹I-radiolabelled anti-Toll-like receptor 5 (TLR5) mAb for non-invasive in vivo graft visualization and quantification in allogeneic transplantation mice model, compared with the non-specific radiotracer ¹⁸F-FDG under using of immunosuppressant. Labelling, binding, and stability studies were performed. BALB/c mice transplanted with C57BL/6 skin grafts, with or without rapamycin treatment (named as allo-treated group or allo-rejection group), were injected with ¹³¹I-anti-TLR5 mAb, ¹⁸F-FDG, or mouse isotype ¹³¹I-IgG, respectively. Whole-body phosphor-autoradiography and ex vivo biodistribution studies were obtained. Whole-body phosphor-autoradiography showed ¹³¹I-anti-TLR5 mAb uptake into organs that were well perfused with blood at 1 hr and showed clear graft images from 12 hrs onwards. The ¹³¹I-anti-TLR5 mAb had significantly higher graft uptake and target-to-non-target ratio in the allo-treated group, as determined by semi-quantification of phosphor-autoradiography images; these results were consistent with ex vivo biodistribution studies. However, high ¹⁸F-FDG uptake was not observed in the allo-treated group. The highest allograft-skin-to-native-skin ratio (A:N) of ¹³¹I-anti-TLR5 mAb uptake was significantly higher than the ratio for ¹⁸F-FDG (7.68 versus 1.16, respectively). ¹³¹I-anti-TLR5 mAb uptake in the grafts significantly correlated with TLR5 expression in the allograft area. The accumulation of ¹³¹I-IgG was comparable in both groups. We conclude that radiolabelled anti-TLR5 mAb is capable of detecting allograft with high target specificity after treatment with the immunosuppressive drug rapamycin.

【 授权许可】

CC BY   
© 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

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