Telocytes in liver regeneration: possible roles

Journal of Cellular and Molecular Medicine

Fei Wang¹  Yang Song¹  Yihua Bei²  Yingying Zhao¹  Junjie Xiao² 

[1] Division of Gastroenterology and Hepatology, Digestive Disease Institute, Tongji Hospital, Tongji University School of Medicine, Shanghai, China;Regeneration Lab and Experimental Center of Life Sciences, School of Life Science, Shanghai University, Shanghai, China

关键词: telocytes;    liver regeneration;    hepatocytes;    CK‐19 positive‐hepatic stem cells;    CD34;    PDGFR‐α;    ß;    EdU;    PCNA;   

DOI  :  10.1111/jcmm.12355

来源: Wiley

【 摘 要 】

Abstract

Telocytes (TCs) are a novel type of interstitial cells which are potentially involved in tissue regeneration and repair (www.telocytes.com). Previously, we documented the presence of TCs in liver. However, the possible roles of TCs in liver regeneration remain unknown. In this study, a murine model of partial hepatectomy (PH) was used to induce liver regeneration. The number of TCs detected by double labelling immunofluorescence methods (CD34/PDGFR-α, CD34/PDGFR-ß and CD34/Vimentin) was significantly increased when a high level of hepatic cell proliferation rate (almost doubled) as shown by 5-ethynyl-2′-deoxyuridine (EdU) immunostaining and Western Blot of Proliferating cell nuclear antigen (PCNA) was found at 48 and 72 hrs post-PH. Meanwhile, the number of CK-19 positive-hepatic stem cells peaked at 72 hrs post-PH, co-ordinating with the same time-point, when the number of TCs was most significantly increased. Taken together, the results indicate a close relationship between TCs and the cells essentially involved in liver regeneration: hepatocytes and stem cells. It remains to be determined how TCs affect hepatocytes proliferation and/or hepatic stem cell differentiation in liver regeneration. Besides intercellular junctions, we may speculate a paracrine effect via ectovesicles.

【 授权许可】

CC BY   
© 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

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