Adrenaline stimulates the proliferation and migration of mesenchymal stem cells towards the LPS‐induced lung injury

Journal of Cellular and Molecular Medicine

Xiaodan Wu¹  Zhiming Wang³  Mengjia Qian²  Lingyan Wang²  Chunxue Bai¹ 

[1] Department of Pulmonary Medicine, Zhongshan Hospital, Fudan University, Shanghai, China;Biomedical Research Center, Zhongshan Hospital, Fudan University, Shanghai, China;Department of Medical Oncology, Zhongshan Hospital, Fudan University, Shanghai, China

关键词: acute lung injury;    mesenchymal stem cells;    lipopolysaccharide;    inflammation;    adrenergic receptor agonists;   

DOI  :  10.1111/jcmm.12283

来源: Wiley

【 摘 要 】

Abstract

Bone marrow-derived mesenchymal stem cells (BMSCs) could modulate inflammation in experimental lung injury. On the other hand, adrenergic receptor agonists could increase DNA synthesis of stem cells. Therefore, we investigated the therapeutic role of adrenaline-stimulated BMSCs on lipopolysaccharide (LPS)-induced lung injury. BMSCs were cultured with adrenergic receptor agonists or antagonists. Suspensions of lung cells or sliced lung tissue from animals with or without LPS-induced injury were co-cultured with BMSCs. LPS-stimulated alveolar macrophages were co-cultured with BMSCs (with adrenaline stimulation or not) in Transwell for 6 hrs. A preliminary animal experiment was conducted to validate the findings in ex vivo study. We found that adrenaline at 10 μM enhanced proliferation of BMSCs through both α- and β-adrenergic receptors. Adrenaline promoted the migration of BMSCs towards LPS-injured lung cells or lung tissue. Adrenaline-stimulated BMSCs decreased the inflammation of LPS-stimulated macrophages, probably through the expression and secretion of several paracrine factors. Adrenaline reduced the extent of injury in LPS-injured rats. Our data indicate that adrenaline-stimulated BMSCs might contribute to the prevention from acute lung injury through the activation of adrenergic receptors, promotion of proliferation and migration towards injured lung, and modulation of inflammation.

【 授权许可】

CC BY   
© 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

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