Journal of Cellular and Molecular Medicine | |
Regional mapping of myocardial hibernation phenotype in idiopathic end‐stage dilated cardiomyopathy | |
Vincenzo Lionetti4  Marco Matteucci4  Marco Ribezzo1  Dario Di Silvestre5  Francesca Brambilla5  Silvia Agostini4  Pierluigi Mauri5  Luigi Padeletti2  Alessandro Pingitore3  Luisa Delsedime6  Mauro Rinaldi1  Fabio A. Recchia4  | |
[1] Cardiac Surgery Department, San Giovanni Battista University Hospital, Turin, Italy;Department of Medical and Surgical Critical Care, University of Florence, Florence, Italy;Institute of Clinical Physiology-CNR, Pisa, Italy;Laboratory of Medical Science, Institute of Life Sciences, Scuola Superiore Sant'Anna, Pisa, Italy;Institute for Biomedical Technologies (ITB)-CNR, Segrate, Milan, Italy;Histopathology Department, S. Giovanni Battista University Hospital, Turin, Italy | |
关键词: pathologic features; hibernating myocardium; chronic heart failure; idiopathic dilated cardiomyopathy; ischaemic microenvironment; nestin; | |
DOI : 10.1111/jcmm.12198 | |
来源: Wiley | |
【 摘 要 】
Myocardial hibernation (MH) is a well-known feature of human ischaemic cardiomyopathy (ICM), whereas its presence in human idiopathic dilated cardiomyopathy (DCM) is still controversial. We investigated the histological and molecular features of MH in left ventricle (LV) regions of failing DCM or ICM hearts. We examined failing hearts from DCM (n = 11; 41.9 ± 5.45 years; left ventricle-ejection fraction (LV-EF), 18 ± 3.16%) and ICM patients (n = 12; 58.08 ± 1.7 years; LVEF, 21.5 ± 6.08%) undergoing cardiac transplantation, and normal donor hearts (N, n = 8). LV inter-ventricular septum (IVS) and antero-lateral free wall (FW) were transmurally (i.e. sub-epicardial, mesocardial and sub-endocardial layers) analysed. LV glycogen content was shown to be increased in both DCM and ICM as compared with N hearts (P < 0.001), with a U-shaped transmural distribution (lower values in mesocardium). Capillary density was homogenously reduced in both DCM and ICM as compared with N (P < 0.05 versus N), with a lower decrease independent of the extent of fibrosis in sub-endocardial and sub-epicardial layers of DCM as compared with ICM. HIF1-α and nestin, recognized ischaemic molecular hallmarks, were similarly expressed in DCM-LV and ICM-LV myocardium. The proteomic profile was overlapping by ~50% in DCM and ICM groups. Morphological and molecular features of MH were detected in end-stage ICM as well as in end-stage DCM LV, despite epicardial coronary artery patency and lower fibrosis in DCM hearts. Unravelling the presence of MH in the absence of coronary stenosis may be helpful to design a novel approach in the clinical management of DCM.Abstract
【 授权许可】
CC BY
© 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
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