Notch4 is activated in endothelial and smooth muscle cells in human brain arteriovenous malformations

Journal of Cellular and Molecular Medicine

Qichuan ZhuGe³  Zhebao Wu³  Lijie Huang³  Bei Zhao³  Ming Zhong³  WeiMing Zheng³  Chen GouRong³  XiaoOu Mao²  Lin Xie²  Xiangdong Wang¹ 

[1] Institute of Clinical & Translational Science, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, China;Buck Institute for Research on Aging, Novato, CA, USA;Zhejiang Provincial Key Laboratory of Aging and Neurological Disorder Research, First Affiliated Hospital, Wenzhou Medical University, Wenzhou, China

关键词: Notch4;    AVM;    human;    brain;    signalling;   

DOI  :  10.1111/jcmm.12115

来源: Wiley

【 摘 要 】

Abstract

Up-regulation of Notch4 was observed in the endothelial cells in the arteriovenous malformations (AVMs) in mice. However, whether Notch4 is also involved in brain AVMs in humans remains unclear. Here, we performed immunohistochemistry on normal brain vascular tissue and surgically resected brain AVMs and found that Notch4 was up-regulated in the subset of abnormal vessels of the brain AVM nidus, compared with control brain vascular tissue. Two-photon confocal images show that Notch4 was expressed not only in the endothelial but also in the smooth muscle cells of the vascular wall in brain AVMs. Western blotting shows that Notch4 was activated in brain AVMs, but not in middle cerebral artery of normal human brain, which was confirmed by immunostaining. Our findings suggest a possible contribution of Notch4 signalling to the development of brain AVMs in human.

【 授权许可】

CC BY   
© 2013 The Authors. Journal of Cellular and Molecular Medicine Published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

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