Circulating plasma serine208‐phosphorylated troponin T levels are indicator of cardiac dysfunction

Journal of Cellular and Molecular Medicine

Emilie Dubois-Deruy²  Aude Belliard²  Paul Mulder¹  Maggy Chwastyniak²  Olivia Beseme²  Jean-Paul Henry¹  Christian Thuillez¹  Philippe Amouyel²  Vincent Richard¹ 

[1] INSERM, U1096, Institute for Biomedical Research, IFRMP 23, Rouen University Hospital, Rouen, France;INSERM, U744, Lille, France

关键词: Rat;    myocardial infarction;    plasma;    biomarker;    phosphorylated troponin T;    ivabradine;    heart failure;   

DOI  :  10.1111/jcmm.12112

来源: Wiley

【 摘 要 】

Abstract

Heart failure (HF) following myocardial infarction (MI) is characterized by progressive alterations of left ventricular (LV) structure and function, named LV remodelling. Although several risk factors such as infarct size have been identified, HF remains difficult to predict in clinical practice. Recently, using phosphoproteomic technology, we found that serine²⁰⁸-phosphorylated troponin T (P-Ser²⁰⁸-TnT) decreases in LV of HF rats. Our aim was to determine the performance of P-Ser²⁰⁸-TnT as plasma biomarker of HF compared to conventional cardiac biomarkers such as B-type natriuretic peptide (BNP), cardiac troponin I (cTnI), C-reactive protein (CRP) or tissue inhibitor of metalloproteinase I (TIMP-1) measured by x-MAP technology, as well as its capacity to reflect a pharmacological improvement of HF. We observed a significant increase of BNP, TnT and cTnI levels and a significant decrease of P-Ser²⁰⁸-TnT and TIMP-1 in the plasma of 2-month-MI rats compared with control rats with no modulation of CRP level. Circulating levels of P-Ser²⁰⁸-TnT were shown to be associated with most of the echocardiographic and haemodynamic parameters of cardiac function. We verified that the decrease of P-Ser²⁰⁸-TnT was not because of an excess of phosphatase activity in plasma of HF rats. Two-month-MI rats treated with the heart rate reducing agent ivabradine had improved LV function and increased plasma levels of P-Ser²⁰⁸-TnT. Thus, circulating phosphorylated troponin T is a highly sensitive biological indicator of cardiac dysfunction and has the potentiality of a new biomarker of HF post-MI, and of a surrogate marker for the efficacy of a successful treatment of HF.

【 授权许可】

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© 2013 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine

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