A logistic model for the detection of circulating tumour cells in human metastatic colorectal cancer

Journal of Cellular and Molecular Medicine

Jorge Barbazán²  María Vieito²  Alicia Abalo²  Lorena Alonso-Alconada²  Laura Muinelo-Romay²  Marta Alonso-Nocelo²  Luís León²  Sonia Candamio²  Elena Gallardo²  Urbano Anido²  Andreas Doll¹  María de los Ángeles Casares³  Antonio Gómez-Tato³  Miguel Abal² 

[1] Biomedical Research Unit, Research Institute Vall d'Hebron University Hospital, Barcelona, Spain;Translational Laboratory, Medical Oncology Department, Complexo Hospitalario Universitario de Santiago de Compostela/SERGAS, Santiago de Compostela, Spain;School of Mathematics, Universidad de Santiago (Campus Vida), Santiago de Compostela, Spain

关键词: metastatic colorectal cancer;    circulating tumour cells;    biomarkers;    GAPDH;    VIL1;    CD45;    logistic model;   

DOI  :  10.1111/j.1582-4934.2012.01544.x

来源: Wiley

【 摘 要 】

Abstract

The accuracy in the diagnosis of metastatic colorectal cancer (mCRC) represents one of the challenges in the clinical management of patients. The detection of circulating tumour cells (CTC) is becoming a promising alternative to current detection techniques, as it focuses on one of the players of the metastatic disease and it should provide with more specific and sensitive detection rates. Here, we describe an improved method of detection of CTC from mCRC patients by combining immune-enrichment, optimal purification of RNA from very low cell numbers, and the selection of accurate PCR probes. As a result, we obtained a logistic model that combines GAPDH and VIL1 normalized to CD45 rendering powerful results in the detection of CTC from mCRC patients (AUROC value 0.8599). We further demonstrated the utility of this model at the clinical setting, as a reliable prognosis tool to determine progression-free survival in mCRC patients. Overall, we developed a strategy that ameliorates the specificity and sensitivity in the detection of CTC, resulting in a robust and promising logistic model for the clinical management of metastatic colorectal cancer patients.

【 授权许可】

Unknown   
© 2012 The Authors Journal of Cellular and Molecular Medicine © 2012 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd