Duodenal expression of iron transport molecules in patients with hereditary hemochromatosis or iron deficiency

Journal of Cellular and Molecular Medicine

Marketa Dostalikova-Cimburova¹  Karolina Kratka³  Kamila Balusikova¹  Jitka Chmelikova¹  Vaclav Hejda⁴  Jan Hnanicek²  Jitka Neubauerova¹  Jana Vranova⁵  Jan Kovar¹ 

[1] Department of Cell and Molecular Biology & Center for Research of Diabetes, Metabolism and Nutrition, Third Faculty of Medicine, Charles University, Prague, Czech Republic;Department of Internal Medicine II, Third Faculty of Medicine, Charles University, Prague, Czech Republic;Department of Internal Medicine I & Center for Research of Diabetes, Metabolism and Nutrition, Third Faculty of Medicine, Charles University, Prague, Czech Republic;1st Department of Medicine, Charles University in Prague, Medical School and Teaching Hospital in Pilsen, Pilsen, Czech Republic;Department of Medical Biophysics and Informatics, Third Faculty of Medicine, Charles University, Prague, Czech Republic

关键词: DMT1;    ferroportin;    Dcytb;    hephaestin;    TFR1;    HFE;    hepcidin;    hemochromatosis;    iron deficiency;    gene expression;   

DOI  :  10.1111/j.1582-4934.2011.01458.x

来源: Wiley

【 摘 要 】

Abstract

Disturbances of iron metabolism are observed in chronic liver diseases. In the present study, we examined gene expression of duodenal iron transport molecules and hepcidin in patients with hereditary hemochromatosis (HHC) (treated and untreated), involving various genotypes (genotypes which represent risk for HHC were examined), and in patients with iron deficiency anaemia (IDA). Gene expressions of DMT1, ferroportin, Dcytb, hephaestin, HFE and TFR1 were measured in duodenal biopsies using real-time PCR and Western blot. Serum hepcidin levels were measured using ELISA. DMT1, ferroportin and TFR1 mRNA levels were significantly increased in post-phlebotomized hemochromatics relative to controls. mRNAs of all tested molecules were significantly increased in patients with IDA compared to controls. The protein expression of ferroportin was increased in both groups of patients but not significantly. Spearman rank correlations showed that DMT1 versus ferroportin, Dcytb versus hephaestin and DMT1 versus TFR1 mRNAs were positively correlated regardless of the underlying cause, similarly to protein levels of ferroportin versus Dcytb and ferroportin versus hephaestin. Serum ferritin was negatively correlated with DMT1 mRNA in investigated groups of patients, except for HHC group. A decrease of serum hepcidin was observed in IDA patients, but this was not statistically significant. Our data showed that although untreated HHC patients do not have increased mRNA levels of iron transport molecules when compared to normal subjects, the expression is relatively increased in relation to body iron stores. On the other hand, post-phlebotomized HHC patients had increased DMT1 and ferroportin mRNA levels possibly due to stimulated erythropoiesis after phlebotomy.

【 授权许可】

Unknown   
© 2012 The Authors Journal of Cellular and Molecular Medicine © 2012 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd