Oncostatin M decreases interleukin‐1 β secretion by human synovial fibroblasts and attenuates an acute inflammatory reaction in vivo

Journal of Cellular and Molecular Medicine

Aline Dumas¹  Stéphanie Lagarde¹  Cynthia Laflamme¹ 

[1] Centre de Recherche en Rhumatologie et Immunologie du CHUQ, and Department of Microbiology-Infectiology and Immunology, Faculty of Medicine, Laval University, Quebec City, QC, Canada

关键词: oncostatin M;    synovial fibroblasts;    neutrophils;    cytokines/chemokines;    inflammation;    murine dorsal air pouch;   

DOI  :  10.1111/j.1582-4934.2011.01412.x

来源: Wiley

【 摘 要 】

Abstract

Oncostatin M (OSM) is a pleiotropic cytokine of the IL-6 family and displays both pro-inflammatory and anti-inflammatory activities. We studied the impact of OSM on the gene activation profile of human synovial cells, which play a central role in the progression of inflammatory responses in joints. In synovial cells stimulated with lipopolysaccharide and recombinant human granulocyte-macrophage colony-stimulating factor, recombinant human OSM and native OSM secreted by human granulocytes both reduced the gene expression and secretion of IL-1β and CXCL8, but increased that of IL-6 and CCL2. This impact on synovial cell activation was not obtained using IL-6 or leukaemia inhibitory factor. Signal transducer and activator of transcription-1 appeared to mediate the effects of OSM on stimulated human synovial fibroblasts. In the murine dorsal air pouch model of inflammation, OSM reduced the expression of the pro-inflammatory cytokines IL-1β and TNF-α in lining tissues, and their presence in the cavity. These results as a whole suggest an anti-inflammatory role for OSM, guiding inflammatory processes towards resolution.

【 授权许可】

Unknown   
© 2011 The Authors Journal compilation © 2011 Foundation for Cellular and Molecular Medicine/Blackwell Publishing Ltd