The therapeutic effects of sodium cromoglycate against influenza A virus H5N1 in mice

Influenza and Other Respiratory Viruses

Deping Han²  Tangting Wei²  Siyi Zhang²  Ming Wang²  Haiyan Tian²  Jinlong Cheng²  Jin Xiao¹  Yanxin Hu² 

[1]Key Laboratory of Veterinary Bioproduction and Chemical Medicine of the Ministry of Agriculture, Zhongmu Institutes of China Animal Husbandry Industry Co., Ltd., Beijing, China

[2]Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, China

关键词: influenza A virus;    sodium cromoglycate;    therapeutic effects;   

DOI  :  10.1111/irv.12334

来源: Wiley

【 摘 要 】

Abstract

Objectives

To identify the protective role of sodium cromoglycate in mice during influenza virus infection.

Design

H5N1 virus-infected mice were treated with the mast cell stabilizer sodium cromoglycate (SCG) to investigate its therapeutic effect.

Sample

The nose, trachea and lungs from mice were collected.

Main outcome measures

Virus replication and host responses were determined by plaque assay, quantitative PCR, immunohistochemistry, and histology.

Results

SCG-treated mice survived better than did PBS-treated mice after H5N1 virus infection. Mild pathological changes with fewer inflammatory cell infiltration and fewer virus antigens were observed in the nose, trachea, and lungs of SCG-treated mice on days 3 and 5 post-infection. However, no significant changes in viral load in the lungs were detected between SCG- and PBS-treated mice. Furthermore, significantly decreased expression of interleukin-6, tumor necrosis factor-a, Toll-like receptor 3, and TIR-domain-containing adapter-inducing interferon-b was detected in the lungs of SCG-treated mice, and no higher expression of interferon-c was detected.

Conclusion

These results suggest that SCG has therapeutic roles in H5N1 virus-infected mice by alleviating the inflammatory response rather than inhibition of viral replication in the lungs.

【 授权许可】

CC BY   
© 2015 The Authors. Influenza and Other Respiratory Viruses Published by John Wiley & Sons Ltd.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.