Midline 1 controls polarization and migration of murine cytotoxic T cells

Immunity, Inflammation and Disease

Lasse Boding³  Ann K. Hansen³  Morten M. Nielsen³  Germana Meroni¹  Thomas H. Braunstein²  Anders Woetmann³  Niels Ødum³  Charlotte M. Bonefeld³ 

[1] Institute for Maternal and Child Health-IRCCS “Burlo Garofolo”, Trieste, Italy;Danish National Research Foundation Centre for Cardiac Arrhythmia, Department of Biomedical Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark;Department of International Health, Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

关键词: contact hypersensitivity;    CTLs;    microtubule;    Midline 1;    migration;   

DOI  :  10.1002/iid3.44

来源: Wiley

【 摘 要 】

Abstract

Midline 1 (MID1) is a microtubule-associated ubiquitin ligase that regulates protein phosphatase 2 A levels. Loss-of-function mutations in MID1 lead to the human X-linked Opitz G/BBB (OS) syndrome characterized by defective midline development during embryogenesis. We have recently shown that MID1 is strongly up-regulated in murine cytotoxic T lymphocytes (CTLs), and that it has a significant impact on exocytosis of lytic granules and the killing capacity of CTLs. The aims of the present study were to determine the localization of MID1 in migrating CTLs, and to investigate whether MID1 affects CTL polarization and migration. We found that MID1 mainly localizes to the uropod of migrating CTLs and that it has a substantial impact on CTL polarization and migration in vitro. Furthermore, analysis of contact hypersensitivity responses supported that MID1 controls effector functions of CTLs in hapten-challenged skin in vivo. These results provide significant new knowledge on the role of MID1 in CTL biology.

【 授权许可】

CC BY   
© 2014 The Authors. Immunity, Inflammation and DiseasePublished by John Wiley & Sons Ltd.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.