Genetic differentiation of the regional Plutella xylostella populations across the Taiwan Strait based on identification of microsatellite markers

Ecology and Evolution

Fushi Ke¹  Shijun You¹  Weiyi He¹  Tiansheng Liu¹  Liette Vasseur¹  Carl J. Douglas¹ 

[1] Institute of Applied Ecology, Fujian Agriculture and Forestry University, Fuzhou, China

关键词: Air currents;    diamondback moth;    gene flow;    genetic variation;    simple sequence repeats;   

DOI  :  10.1002/ece3.1850

来源: Wiley

【 摘 要 】

Abstract

Movement of individuals through events, such as storms or crop transportation, may affect survival and distribution of insect pests, as well as population genetic structure at a regional scale. Understanding what factors contribute to gene flow in pest populations remains very important for sustainable pest management. The diamondback moth (Plutella xylostella) is an insect pest well known for its capacity of moving over short to long distances. Here, we used newly isolated microsatellite markers to analyze the genetic structure of nine populations across the Taiwan Strait of China (Taiwan and Fujian). A total of 12,152 simple sequence repeats (SSRs) were initially identified from the P. xylostella transcriptome (~94 Mb), with an average of 129 SSRs per Mb. Nine SSRs were validated to be polymorphic markers, and eight were used for this population genetic study. Our results showed that the P. xylostella populations could be divided into distinct two clusters, which is likely due to the year-round airflows in this region. A pattern of isolation by distance among the local populations within Fujian was found, and may be related to vegetable transportation. Considering the complexity of the P. xylostella population genetic structure from local and regional to global levels, we propose that developing ecologically sound strategies for managing this pest will require knowledge of the link between behavioral and population ecology and its genetic structure.

【 授权许可】

CC BY   
© 2015 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.