Nutrient cycling in early coral life stages: Pocillopora damicornis larvae provide their algal symbiont (Symbiodinium) with nitrogen acquired from bacterial associates

Ecology and Evolution

Janja Ceh¹  Matt R. Kilburn³  John B. Cliff³  Jean-Baptiste Raina¹  Mike van Keulen² 

[1] Australian Institute of Marine Science, Townsville, Australia;School of Veterinary and Life Sciences, Murdoch University, Murdoch, WA, Australia;Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Crawley, WA 6009, Australia

关键词: Bacteria;    coral larvae;    nanoSIMS;    nitrogen;   

DOI  :  10.1002/ece3.642

来源: Wiley

【 摘 要 】

Abstract

The waters surrounding coral reef ecosystems are generally poor in nutrients, yet their levels of primary production are comparable with those reported from tropical rain forests. One explanation of this paradox is the efficient cycling of nutrients between the coral host, its endosymbiotic alga Symbiodinium and a wide array of microorganisms. Despite their importance for the animals' fitness, the cycling of nutrients in early coral life stages and the initial establishment of partnerships with the microbes involved in these processes has received little scrutiny to date. Nitrogen is an essential but limited nutrient in coral reef ecosystems. In order to assess the early nutrient exchange between bacteria and corals, coral larvae of the species Pocillopora damicornis were incubated with two coral-associated bacteria (Alteromonas sp., or Vibrio alginolyticus), prelabeled with the stable nitrogen isotope ¹⁵N. The incorporation and translocation of nitrogen from Vibrio- and Alteromonas bacteria into P. damicornis coral larvae and specifically into the coral-symbiotic Symbiodinium were detected by nanoscale secondary ion mass spectrometry (NanoSIMS). A significant increase in the amount of enriched ¹⁵N (two to threefold compared to natural abundance) was observed in P. damicornis larvae within 8 h of incubation for both bacterial treatments (one-way ANOVA, F_5,53 = 18.03, P = 0.004 for Alteromonas sp. and F_5,53 = 18.03, P = 0.0001 for V. alginolyticus). These findings reveal that coral larvae acquire nutrients previously taken up from the environment by bacteria. The additional nitrogen may increase the survival rate and fitness of the developing coral and therefore contribute to the successful maintenance of coral reefs.

【 授权许可】

CC BY   
© 2013 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.