Validation of microsatellite multiplexes for parentage analysis and species discrimination in two hybridizing species of coral reef fish (Plectropomus spp., Serranidae)

Ecology and Evolution

Hugo B. Harrison³  Kevin A. Feldheim⁴  Geoffrey P. Jones⁵  Kayan Ma²  Hicham Mansour¹  Sadhasivam Perumal¹  David H. Williamson⁵ 

[1] Biosciences Core Laboratory, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia;School of Marine and Tropical Biology, James Cook University, Townsville, Queensland, Australia;ORCID:orcid.org/0000-0001-8831-0086;Pritzker Laboratory for Molecular Systematics and Evolution, The Field Museum, Chicago, Illinois;Centre of Excellence for Coral Reef Studies, James Cook University, Townsville, Queensland, Australia

关键词: coral trout;    hybridization;    microsatellite multiplex;    parentage analysis;    Plectropomus spp;   

DOI  :  10.1002/ece3.1002

来源: Wiley

【 摘 要 】

Abstract

Microsatellites are often considered ideal markers to investigate ecological processes in animal populations. They are regularly used as genetic barcodes to identify species, individuals, and infer familial relationships. However, such applications are highly sensitive the number and diversity of microsatellite markers, which are also prone to error. Here, we propose a novel framework to assess the suitability of microsatellite datasets for parentage analysis and species discrimination in two closely related species of coral reef fish, Plectropomus leopardus and P. maculatus (Serranidae). Coral trout are important fisheries species throughout the Indo-Pacific region and have been shown to hybridize in parts of the Great Barrier Reef, Australia. We first describe the development of 25 microsatellite loci and their integration to three multiplex PCRs that co-amplify in both species. Using simulations, we demonstrate that the complete suite of markers provides appropriate power to discriminate between species, detect hybrid individuals, and resolve parent–offspring relationships in natural populations, with over 99.6% accuracy in parent–offspring assignments. The markers were also tested on seven additional species within the Plectropomus genus with polymorphism in 28–96% of loci. The multiplex PCRs developed here provide a reliable and cost-effective strategy to investigate evolutionary and ecological dynamics and will be broadly applicable in studies of wild populations and aquaculture brood stocks for these closely related fish species.

【 授权许可】

CC BY   
© 2014 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.